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6X1J
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BU of 6x1j by Molmil
The homing endonuclease I-WcaI bound to its DNA recognition sequence
Descriptor: DNA (25-mer), POTASSIUM ION, Probable intron-encoded endonuclease 1
Authors:Nawimanage, R, Lohman, J.R, Gimble, F.S.
Deposit date:2020-05-19
Release date:2021-05-26
Last modified:2023-10-18
Method:X-RAY DIFFRACTION (1.945 Å)
Cite:Structure-Function Studies of Two Yeast Homing Endonucleases that Evolved to Cleave Identical Targets with Dissimilar Rates and Specificities.
J.Mol.Biol., 434, 2022
1R7M
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BU of 1r7m by Molmil
The homing endonuclease I-SceI bound to its DNA recognition region
Descriptor: 5'-D(*CP*AP*CP*GP*CP*TP*AP*GP*GP*GP*AP*TP*AP*AP*CP*AP*GP*GP*GP*TP*AP*AP*TP*AP*C)-3', 5'-D(*GP*GP*TP*AP*TP*TP*AP*CP*CP*CP*TP*GP*TP*TP*AP*TP*CP*CP*CP*TP*AP*GP*CP*GP*T)-3', CALCIUM ION, ...
Authors:Moure, C.M, Gimble, F.S, Quiocho, F.A.
Deposit date:2003-10-21
Release date:2004-10-26
Last modified:2024-02-14
Method:X-RAY DIFFRACTION (2.25 Å)
Cite:The crystal structure of the gene targeting homing endonuclease I-SceI reveals the origins of its target site specificity
J.Mol.Biol., 334, 2003
1LWS
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BU of 1lws by Molmil
Crystal structure of the intein homing endonuclease PI-SceI bound to its recognition sequence
Descriptor: CALCIUM ION, ENDONUCLEASE PI-SCEI, PI-SceI DNA recognition region bottom strand, ...
Authors:Moure, C.M, Gimble, F.S, Quiocho, F.A.
Deposit date:2002-06-03
Release date:2002-09-27
Last modified:2011-07-13
Method:X-RAY DIFFRACTION (3.5 Å)
Cite:Crystal structure of the intein homing endonuclease PI-SceI bound to its recognition sequence.
Nat.Struct.Biol., 9, 2002
1LWT
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BU of 1lwt by Molmil
Crystal structure of the intein homing endonuclease PI-SceI bound to its substrate DNA (Ca2+ free)
Descriptor: ENDONUCLEASE PI-SCEI, PI-SceI DNA substrate bottom strand, PI-SceI DNA substrate top strand
Authors:Moure, C.M, Gimble, F.S, Quiocho, F.A.
Deposit date:2002-06-03
Release date:2002-09-27
Last modified:2011-07-13
Method:X-RAY DIFFRACTION (3.2 Å)
Cite:Crystal structure of the intein homing endonuclease PI-SceI bound to its recognition sequence.
Nat.Struct.Biol., 9, 2002
1DFA
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BU of 1dfa by Molmil
CRYSTAL STRUCTURE OF PI-SCEI IN C2 SPACE GROUP
Descriptor: PI-SCEI ENDONUCLEASE
Authors:Hu, D, Crist, M, Duan, X, Quiocho, F.A, Gimble, F.S.
Deposit date:1999-11-18
Release date:1999-12-08
Last modified:2024-02-07
Method:X-RAY DIFFRACTION (2 Å)
Cite:Probing the structure of the PI-SceI-DNA complex by affinity cleavage and affinity photocross-linking.
J.Biol.Chem., 275, 2000
3C0X
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BU of 3c0x by Molmil
I-SceI in complex with a top nicked DNA substrate
Descriptor: CALCIUM ION, DNA (5'-D(*DC*DAP*DCP*DGP*DCP*DTP*DAP*DGP*DGP*DGP*DAP*DTP*DAP*DA)-3'), DNA (5'-D(*DG*DGP*DTP*DAP*DTP*DTP*DAP*DCP*DCP*DCP*DTP*DGP*DTP*DTP*DAP*DTP*DCP*DCP*DCP*DTP*DAP*DGP*DCP*DGP*DT)-3'), ...
Authors:Moure, C.M, Gimble, F.S, Quiocho, F.A.
Deposit date:2008-01-21
Release date:2008-05-06
Last modified:2023-08-30
Method:X-RAY DIFFRACTION (2.3 Å)
Cite:Crystal structures of I-SceI complexed to nicked DNA substrates: snapshots of intermediates along the DNA cleavage reaction pathway.
Nucleic Acids Res., 36, 2008
3C0W
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BU of 3c0w by Molmil
I-SceI in complex with a bottom nicked DNA substrate
Descriptor: CALCIUM ION, DNA (5'-D(*DAP*DCP*DGP*DCP*DTP*DAP*DGP*DGP*DGP*DAP*DTP*DAP*DAP*DCP*DAP*DGP*DGP*DGP*DTP*DAP*DAP*DTP*DAP*DC)-3'), DNA (5'-D(*DGP*DTP*DAP*DTP*DTP*DAP*DCP*DCP*DCP*DTP*DGP*DTP*DTP*DAP*DT)-3'), ...
Authors:Moure, C.M, Gimble, F.S, Quiocho, F.A.
Deposit date:2008-01-21
Release date:2008-05-06
Last modified:2023-08-30
Method:X-RAY DIFFRACTION (2.2 Å)
Cite:Crystal structures of I-SceI complexed to nicked DNA substrates: snapshots of intermediates along the DNA cleavage reaction pathway.
Nucleic Acids Res., 36, 2008
3OOR
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BU of 3oor by Molmil
I-SceI mutant (K86R/G100T)complexed with C/G+4 DNA substrate
Descriptor: 5'-D(*CP*AP*CP*GP*CP*TP*AP*GP*GP*GP*AP*TP*AP*AP*CP*CP*GP*GP*GP*TP*AP*AP*TP*AP*C)-3', 5'-D(*GP*GP*TP*AP*TP*TP*AP*CP*CP*CP*GP*GP*TP*TP*AP*TP*CP*CP*CP*TP*AP*GP*CP*GP*T)-3', CALCIUM ION, ...
Authors:Joshi, R, Chen, J.-H, Golden, B.L, Gimble, F.S.
Deposit date:2010-08-31
Release date:2010-11-17
Last modified:2023-09-06
Method:X-RAY DIFFRACTION (2.5 Å)
Cite:Evolution of I-SceI Homing Endonucleases with Increased DNA Recognition Site Specificity.
J.Mol.Biol., 405, 2011
3OOL
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BU of 3ool by Molmil
I-SceI complexed with C/G+4 DNA substrate
Descriptor: 5'-D(*CP*AP*CP*GP*CP*TP*AP*GP*GP*GP*AP*TP*AP*AP*CP*CP*GP*GP*GP*TP*AP*AP*TP*AP*C)-3', 5'-D(*GP*GP*TP*AP*TP*TP*AP*CP*CP*CP*GP*GP*TP*TP*AP*TP*CP*CP*CP*TP*AP*GP*CP*GP*T)-3', CALCIUM ION, ...
Authors:Joshi, R, Chen, J.-H, Golden, B.L, Gimble, F.S.
Deposit date:2010-08-31
Release date:2010-11-17
Last modified:2023-09-06
Method:X-RAY DIFFRACTION (2.3 Å)
Cite:Evolution of I-SceI Homing Endonucleases with Increased DNA Recognition Site Specificity.
J.Mol.Biol., 405, 2011
1VDE
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BU of 1vde by Molmil
PI-SCEI, A HOMING ENDONUCLEASE WITH PROTEIN SPLICING ACTIVITY
Descriptor: PI-SCEI
Authors:Duan, X, Quiocho, F.A.
Deposit date:1997-04-01
Release date:1998-04-08
Last modified:2024-02-14
Method:X-RAY DIFFRACTION (2.4 Å)
Cite:Crystal structure of PI-SceI, a homing endonuclease with protein splicing activity.
Cell(Cambridge,Mass.), 89, 1997
1EF0
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BU of 1ef0 by Molmil
CRYSTAL STRUCTURE OF PI-SCEI MINIPRECURSOR
Descriptor: PI-SCEI ENDONUCLEASE, ZINC ION
Authors:Poland, B.W, Xu, M.-Q, Quiocho, F.A.
Deposit date:2000-02-04
Release date:2000-06-07
Last modified:2024-02-07
Method:X-RAY DIFFRACTION (2.1 Å)
Cite:Structural insights into the protein splicing mechanism of PI-SceI.
J.Biol.Chem., 275, 2000

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