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6M0D
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BU of 6m0d by Molmil
Beijerinckia indica beta-fructosyltransferase
Descriptor: Levansucrase, MAGNESIUM ION
Authors:Tonozuka, T.
Deposit date:2020-02-21
Release date:2020-08-12
Last modified:2023-11-29
Method:X-RAY DIFFRACTION (2.2 Å)
Cite:Crystal structure of a glycoside hydrolase family 68 beta-fructosyltransferase from Beijerinckia indica subsp. indica in complex with fructose.
Biosci.Biotechnol.Biochem., 84, 2020
6M0E
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BU of 6m0e by Molmil
Beijerinckia indica beta-fructosyltransferase complexed with fructose
Descriptor: Levansucrase, MAGNESIUM ION, beta-D-fructofuranose, ...
Authors:Tonozuka, T.
Deposit date:2020-02-21
Release date:2020-08-12
Last modified:2023-11-29
Method:X-RAY DIFFRACTION (1.35 Å)
Cite:Crystal structure of a glycoside hydrolase family 68 beta-fructosyltransferase from Beijerinckia indica subsp. indica in complex with fructose.
Biosci.Biotechnol.Biochem., 84, 2020
5B6S
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BU of 5b6s by Molmil
Catalytic domain of Coprinopsis cinerea GH62 alpha-L-arabinofuranosidase
Descriptor: CALCIUM ION, GLYCEROL, Glycosyl hydrolase family 62 protein
Authors:Tonozuka, T.
Deposit date:2016-06-01
Release date:2016-09-07
Last modified:2023-11-08
Method:X-RAY DIFFRACTION (1.7 Å)
Cite:Structure of the Catalytic Domain of alpha-L-Arabinofuranosidase from Coprinopsis cinerea, CcAbf62A, Provides Insights into Structure-Function Relationships in Glycoside Hydrolase Family 62
Appl. Biochem. Biotechnol., 181, 2017
5B6T
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BU of 5b6t by Molmil
Catalytic domain of Coprinopsis cinerea GH62 alpha-L-arabinofuranosidase complexed with Pb
Descriptor: CALCIUM ION, GLYCEROL, Glycosyl hydrolase family 62 protein, ...
Authors:Tonozuka, T.
Deposit date:2016-06-01
Release date:2016-09-07
Last modified:2023-11-08
Method:X-RAY DIFFRACTION (1.48 Å)
Cite:Structure of the Catalytic Domain of alpha-L-Arabinofuranosidase from Coprinopsis cinerea, CcAbf62A, Provides Insights into Structure-Function Relationships in Glycoside Hydrolase Family 62
Appl. Biochem. Biotechnol., 181, 2017
5Z0U
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BU of 5z0u by Molmil
Thermoactinomyces vulgaris R-47 alpha-amylase I (TVA I) 11 residues (from A363 to N373) deletion mutant (Del11)
Descriptor: (4S)-2-METHYL-2,4-PENTANEDIOL, CALCIUM ION, Neopullulanase 1
Authors:Tonozuka, T.
Deposit date:2017-12-21
Release date:2018-02-21
Last modified:2023-11-22
Method:X-RAY DIFFRACTION (1.37 Å)
Cite:Mutagenesis-induced conformational change in domain B of a pullulan-hydrolyzing alpha-amylase TVA I
Amylase, 2, 2018
5Z0T
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BU of 5z0t by Molmil
Thermoactinomyces vulgaris R-47 alpha-amylase I (TVA I) mutant A357V/Q359N/Y360E (AQY/VNE)
Descriptor: (4S)-2-METHYL-2,4-PENTANEDIOL, CALCIUM ION, Neopullulanase 1
Authors:Tonozuka, T.
Deposit date:2017-12-21
Release date:2018-02-21
Last modified:2023-11-22
Method:X-RAY DIFFRACTION (1.5 Å)
Cite:Mutagenesis-induced conformational change in domain B of a pullulan-hydrolyzing alpha-amylase TVA I
Amylase, 2, 2018
2ZYK
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BU of 2zyk by Molmil
Crystal structure of cyclo/maltodextrin-binding protein complexed with gamma-cyclodextrin
Descriptor: Cyclooctakis-(1-4)-(alpha-D-glucopyranose), Solute-binding protein
Authors:Tonozuka, T, Sogawa, A, Yamada, M, Matsumoto, N, Yoshida, H, Kamitori, S, Ichikawa, K, Mizuno, M, Nishikawa, A, Sakano, Y.
Deposit date:2009-01-26
Release date:2009-02-10
Last modified:2024-03-13
Method:X-RAY DIFFRACTION (2.5 Å)
Cite:Structural basis for cyclodextrin recognition by Thermoactinomyces vulgaris cyclo/maltodextrin-binding protein
Febs J., 274, 2007
3VSS
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BU of 3vss by Molmil
Microbacterium saccharophilum K-1 beta-fructofuranosidase catalytic domain complexed with fructose
Descriptor: Beta-fructofuranosidase, beta-D-fructofuranose
Authors:Tonozuka, T, Tamaki, A, Yokoi, G, Miyazaki, T, Ichikawa, M, Nishikawa, A, Ohta, Y, Hidaka, Y, Katayama, K, Hatada, Y, Ito, T, Fujita, K.
Deposit date:2012-05-08
Release date:2012-08-22
Last modified:2023-11-08
Method:X-RAY DIFFRACTION (1.97 Å)
Cite:Crystal structure of a lactosucrose-producing enzyme, Arthrobacter sp. K-1 beta-fructofuranosidase
Enzyme.Microb.Technol., 51, 2012
3VSR
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BU of 3vsr by Molmil
Microbacterium saccharophilum K-1 beta-fructofuranosidase catalytic domain
Descriptor: Beta-fructofuranosidase
Authors:Tonozuka, T, Tamaki, A, Yokoi, G, Miyazaki, T, Ichikawa, M, Nishikawa, A, Ohta, Y, Hidaka, Y, Katayama, K, Hatada, Y, Ito, T, Fujita, K.
Deposit date:2012-05-08
Release date:2012-08-22
Last modified:2023-11-08
Method:X-RAY DIFFRACTION (2 Å)
Cite:Crystal structure of a lactosucrose-producing enzyme, Arthrobacter sp. K-1 beta-fructofuranosidase
Enzyme.Microb.Technol., 51, 2012
8XX9
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BU of 8xx9 by Molmil
Rhodothermus marinus alpha-amylase RmGH13_47A CBM48-A-B-C domains
Descriptor: (4S)-2-METHYL-2,4-PENTANEDIOL, 2-(N-MORPHOLINO)-ETHANESULFONIC ACID, CALCIUM ION, ...
Authors:Tonozuka, T.
Deposit date:2024-01-18
Release date:2024-02-07
Method:X-RAY DIFFRACTION (1.55 Å)
Cite:Structural basis for the recognition of alpha-1,6-branched alpha-glucan by GH13_47 alpha-amylase from Rhodothermus marinus
To Be Published
8XXA
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BU of 8xxa by Molmil
Rhodothermus marinus alpha-amylase RmGH13_47A CBM48-A-B-C domains in complex with branched pentasaccharide
Descriptor: 2-(N-MORPHOLINO)-ETHANESULFONIC ACID, CALCIUM ION, DI(HYDROXYETHYL)ETHER, ...
Authors:Tonozuka, T.
Deposit date:2024-01-18
Release date:2024-02-07
Method:X-RAY DIFFRACTION (1.55 Å)
Cite:Structural basis for the recognition of alpha-1,6-branched alpha-glucan by GH13_47 alpha-amylase from Rhodothermus marinus
To Be Published
7XOI
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BU of 7xoi by Molmil
Aspergillus sojae alpha-glucosidase AsojAgdL in complex with trehalose
Descriptor: 2-acetamido-2-deoxy-beta-D-glucopyranose, 2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose, SODIUM ION, ...
Authors:Tonozuka, T.
Deposit date:2022-05-01
Release date:2022-06-15
Last modified:2023-11-29
Method:X-RAY DIFFRACTION (2.3 Å)
Cite:Structural basis for proteolytic processing of Aspergillus sojae alpha-glucosidase L with strong transglucosylation activity.
J.Struct.Biol., 214, 2022
8I2Q
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BU of 8i2q by Molmil
Beijerinckia indica beta-fructosyltransferase variant H395R/F473Y
Descriptor: Beta-fructosyltransferase, GLYCEROL
Authors:Tonozuka, T.
Deposit date:2023-01-15
Release date:2023-06-14
Last modified:2023-09-06
Method:X-RAY DIFFRACTION (2.15 Å)
Cite:Characterization and alteration of product specificity of Beijerinckia indica subsp. indica beta-fructosyltransferase.
Biosci.Biotechnol.Biochem., 87, 2023
8I2R
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BU of 8i2r by Molmil
Beijerinckia indica beta-fructosyltransferase variant H395R/F473Y in complex with fructose
Descriptor: Beta-fructosyltransferase, MAGNESIUM ION, beta-D-fructofuranose, ...
Authors:Tonozuka, T.
Deposit date:2023-01-15
Release date:2023-06-14
Last modified:2023-09-06
Method:X-RAY DIFFRACTION (1.36 Å)
Cite:Characterization and alteration of product specificity of Beijerinckia indica subsp. indica beta-fructosyltransferase.
Biosci.Biotechnol.Biochem., 87, 2023
7VCP
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BU of 7vcp by Molmil
Frischella perrara beta-fructofuranosidase in complex with fructose
Descriptor: DI(HYDROXYETHYL)ETHER, PENTAETHYLENE GLYCOL, Sucrose-6-phosphate hydrolase, ...
Authors:Tonozuka, T.
Deposit date:2021-09-03
Release date:2022-03-09
Last modified:2023-11-29
Method:X-RAY DIFFRACTION (2 Å)
Cite:Enzymatic and structural characterization of beta-fructofuranosidase from the honeybee gut bacterium Frischella perrara.
Appl.Microbiol.Biotechnol., 106, 2022
7VCO
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BU of 7vco by Molmil
Frischella perrara beta-fructofuranosidase
Descriptor: DI(HYDROXYETHYL)ETHER, Sucrose-6-phosphate hydrolase, TRIETHYLENE GLYCOL
Authors:Tonozuka, T.
Deposit date:2021-09-03
Release date:2022-03-09
Last modified:2023-11-29
Method:X-RAY DIFFRACTION (1.6 Å)
Cite:Enzymatic and structural characterization of beta-fructofuranosidase from the honeybee gut bacterium Frischella perrara.
Appl.Microbiol.Biotechnol., 106, 2022
6JR6
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BU of 6jr6 by Molmil
Flavobacterium johnsoniae GH31 dextranase, FjDex31A
Descriptor: (4S)-2-METHYL-2,4-PENTANEDIOL, ACETATE ION, Candidate alpha-glycosidase Glycoside hydrolase family 31
Authors:Tonozuka, T.
Deposit date:2019-04-02
Release date:2019-04-24
Last modified:2020-04-01
Method:X-RAY DIFFRACTION (2 Å)
Cite:Structural insights into polysaccharide recognition by Flavobacterium johnsoniae dextranase, a member of glycoside hydrolase family 31.
Febs J., 287, 2020
6JR7
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BU of 6jr7 by Molmil
Flavobacterium johnsoniae GH31 dextranase, FjDex31A, complexed with glucose
Descriptor: (4S)-2-METHYL-2,4-PENTANEDIOL, ACETATE ION, Candidate alpha-glycosidase Glycoside hydrolase family 31, ...
Authors:Tonozuka, T.
Deposit date:2019-04-02
Release date:2019-04-24
Last modified:2023-11-22
Method:X-RAY DIFFRACTION (1.75 Å)
Cite:Structural insights into polysaccharide recognition by Flavobacterium johnsoniae dextranase, a member of glycoside hydrolase family 31.
Febs J., 287, 2020
6JR8
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BU of 6jr8 by Molmil
Flavobacterium johnsoniae GH31 dextranase, FjDex31A, mutant D412A complexed with isomaltotriose
Descriptor: (4S)-2-METHYL-2,4-PENTANEDIOL, ACETATE ION, Candidate alpha-glycosidase Glycoside hydrolase family 31, ...
Authors:Tonozuka, T.
Deposit date:2019-04-02
Release date:2019-04-24
Last modified:2023-11-22
Method:X-RAY DIFFRACTION (1.8 Å)
Cite:Structural insights into polysaccharide recognition by Flavobacterium johnsoniae dextranase, a member of glycoside hydrolase family 31.
Febs J., 287, 2020
5AWQ
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BU of 5awq by Molmil
Arthrobacter globiformis T6 isomalto-dextranse complexed with panose
Descriptor: Isomaltodextranase, PHOSPHATE ION, alpha-D-glucopyranose-(1-6)-alpha-D-glucopyranose, ...
Authors:Tonozuka, T.
Deposit date:2015-07-08
Release date:2015-09-09
Last modified:2020-07-29
Method:X-RAY DIFFRACTION (1.48 Å)
Cite:Crystal Structure and Mutational Analysis of Isomalto-dextranase, a Member of Glycoside Hydrolase Family 27
J.Biol.Chem., 290, 2015
5AWP
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BU of 5awp by Molmil
Arthrobacter globiformis T6 isomalto-dextranase complexed with isomaltose
Descriptor: Isomaltodextranase, PHOSPHATE ION, alpha-D-glucopyranose-(1-6)-beta-D-glucopyranose
Authors:Tonozuka, T.
Deposit date:2015-07-08
Release date:2015-09-09
Last modified:2023-11-08
Method:X-RAY DIFFRACTION (2 Å)
Cite:Crystal Structure and Mutational Analysis of Isomalto-dextranase, a Member of Glycoside Hydrolase Family 27
J.Biol.Chem., 290, 2015
5AWO
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BU of 5awo by Molmil
Arthrobacter globiformis T6 isomalto-dextranse
Descriptor: ACETATE ION, Isomaltodextranase, PHOSPHATE ION
Authors:Tonozuka, T.
Deposit date:2015-07-08
Release date:2015-09-09
Last modified:2020-02-26
Method:X-RAY DIFFRACTION (1.44 Å)
Cite:Crystal Structure and Mutational Analysis of Isomalto-dextranase, a Member of Glycoside Hydrolase Family 27
J.Biol.Chem., 290, 2015
1BVZ
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BU of 1bvz by Molmil
ALPHA-AMYLASE II (TVAII) FROM THERMOACTINOMYCES VULGARIS R-47
Descriptor: PROTEIN (ALPHA-AMYLASE II)
Authors:Kamitori, S, Kondo, S, Okuyama, K, Yokota, T, Shimura, Y, Tonozuka, T, Sakano, Y.
Deposit date:1998-09-22
Release date:1999-03-02
Last modified:2024-02-07
Method:X-RAY DIFFRACTION (2.6 Å)
Cite:Crystal structure of Thermoactinomyces vulgaris R-47 alpha-amylase II (TVAII) hydrolyzing cyclodextrins and pullulan at 2.6 A resolution.
J.Mol.Biol., 287, 1999
4WVC
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BU of 4wvc by Molmil
Crystal structure of GH63 mannosylglycerate hydrolase from Thermus thermophilus HB8 in complex with Tris and D-glycerate
Descriptor: (2R)-2,3-DIHYDROXYPROPANOIC ACID, 2-AMINO-2-HYDROXYMETHYL-PROPANE-1,3-DIOL, CHLORIDE ION, ...
Authors:Miyazaki, T, Ichikawa, M, Nishikawa, A, Tonozuka, T.
Deposit date:2014-11-05
Release date:2015-03-11
Last modified:2024-03-20
Method:X-RAY DIFFRACTION (2.1 Å)
Cite:Crystal structure and substrate-binding mode of GH63 mannosylglycerate hydrolase from Thermus thermophilus HB8.
J.Struct.Biol., 190, 2015
4WVA
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BU of 4wva by Molmil
Crystal structure of GH63 mannosylglycerate hydrolase from Thermus thermophilus HB8 in complex with Tris
Descriptor: (4S)-2-METHYL-2,4-PENTANEDIOL, 2-AMINO-2-HYDROXYMETHYL-PROPANE-1,3-DIOL, CHLORIDE ION, ...
Authors:Miyazaki, T, Ichikawa, M, Nishikawa, A, Tonozuka, T.
Deposit date:2014-11-05
Release date:2015-03-11
Last modified:2023-11-08
Method:X-RAY DIFFRACTION (1.67 Å)
Cite:Crystal structure and substrate-binding mode of GH63 mannosylglycerate hydrolase from Thermus thermophilus HB8.
J.Struct.Biol., 190, 2015

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