HEADER    TRANSLATION                             05-SEP-07   2VAZ              
TITLE     MODEL OF THE S15-MRNA COMPLEX FITTED INTO THE CRYO-EM MAP OF THE 70S  
TITLE    2 ENTRAPMENT COMPLEX.                                                  
COMPND    MOL_ID: 1;                                                            
COMPND   2 MOLECULE: RPSO MRNA OPERATOR;                                        
COMPND   3 CHAIN: A;                                                            
COMPND   4 FRAGMENT: HAIRPIN LOOP DOMAIN I AND PSEUDOKNOT;                      
COMPND   5 ENGINEERED: YES;                                                     
COMPND   6 MOL_ID: 2;                                                           
COMPND   7 MOLECULE: 30S RIBOSOMAL PROTEIN S15;                                 
COMPND   8 CHAIN: F;                                                            
COMPND   9 SYNONYM: REPRESSOR S15;                                              
COMPND  10 ENGINEERED: YES                                                      
SOURCE    MOL_ID: 1;                                                            
SOURCE   2 SYNTHETIC: YES;                                                      
SOURCE   3 ORGANISM_SCIENTIFIC: ESCHERICHIA COLI;                               
SOURCE   4 ORGANISM_TAXID: 562;                                                 
SOURCE   5 MOL_ID: 2;                                                           
SOURCE   6 ORGANISM_SCIENTIFIC: ESCHERICHIA COLI;                               
SOURCE   7 ORGANISM_TAXID: 562;                                                 
SOURCE   8 EXPRESSION_SYSTEM: ESCHERICHIA COLI;                                 
SOURCE   9 EXPRESSION_SYSTEM_TAXID: 469008;                                     
SOURCE  10 EXPRESSION_SYSTEM_STRAIN: BL21(DE3);                                 
SOURCE  11 EXPRESSION_SYSTEM_PLASMID: PET11C                                    
KEYWDS    TRANSLATION, PLATFORM-BINDING CENTER, GENE EXPRESSION REGULATION,     
KEYWDS   2 RIBOSOMAL PROTEIN, RIBONUCLEOPROTEIN, PROTEIN SYNTHESIS, TRANSLATION 
KEYWDS   3 INITIATION, RIBOSOME, RIBOSWITCH, RNA-BINDING, RRNA-BINDING, RNA     
KEYWDS   4 PSEUDOKNOT, MRNA STRUCTURE, REPRESSOR PROTEIN                        
EXPDTA    ELECTRON MICROSCOPY                                                   
MDLTYP    P ATOMS ONLY, CHAIN A ; CA ATOMS ONLY, CHAIN F                        
AUTHOR    S.MARZI,A.G.MYASNIKOV,A.SERGANOV,C.EHRESMANN,P.ROMBY,M.YUSUPOV,       
AUTHOR   2 B.P.KLAHOLZ                                                          
REVDAT   4   02-AUG-17 2VAZ    1                                                
REVDAT   3   14-NOV-12 2VAZ    1       REMARK VERSN                             
REVDAT   2   24-FEB-09 2VAZ    1       VERSN                                    
REVDAT   1   02-OCT-07 2VAZ    0                                                
JRNL        AUTH   S.MARZI,A.G.MYASNIKOV,A.SERGANOV,C.EHRESMANN,P.ROMBY,        
JRNL        AUTH 2 M.YUSUPOV,B.P.KLAHOLZ                                        
JRNL        TITL   STRUCTURED MRNAS REGULATE TRANSLATION INITIATION BY BINDING  
JRNL        TITL 2 TO THE PLATFORM OF THE RIBOSOME.                             
JRNL        REF    CELL(CAMBRIDGE,MASS.)         V. 130  1019 2007              
JRNL        REFN                   ISSN 0092-8674                               
JRNL        PMID   17889647                                                     
JRNL        DOI    10.1016/J.CELL.2007.07.008                                   
REMARK   2                                                                      
REMARK   2 RESOLUTION.   10.00 ANGSTROMS.                                       
REMARK   3                                                                      
REMARK   3 REFINEMENT.                                                          
REMARK   3   SOFTWARE PACKAGES      : O, PYMOL, IMAGIC                          
REMARK   3   RECONSTRUCTION SCHEMA  : NULL                                      
REMARK   3                                                                      
REMARK   3 EM MAP-MODEL FITTING AND REFINEMENT                                  
REMARK   3   PDB ENTRY                    : 2AW7                                
REMARK   3   REFINEMENT SPACE             : REAL                                
REMARK   3   REFINEMENT PROTOCOL          : OTHER                               
REMARK   3   REFINEMENT TARGET            : NULL                                
REMARK   3   OVERALL ANISOTROPIC B VALUE  : NULL                                
REMARK   3                                                                      
REMARK   3 FITTING PROCEDURE : METHOD--MANUAL REFINEMENT PROTOCOL--X-RAY AND    
REMARK   3  HOMOLOGY MODELS                                                     
REMARK   3                                                                      
REMARK   3 EM IMAGE RECONSTRUCTION STATISTICS                                   
REMARK   3   NOMINAL PIXEL SIZE (ANGSTROMS)    : 3.000                          
REMARK   3   ACTUAL PIXEL SIZE  (ANGSTROMS)    : NULL                           
REMARK   3   EFFECTIVE RESOLUTION (ANGSTROMS)  : 10.00                          
REMARK   3   NUMBER OF PARTICLES               : 31415                          
REMARK   3   CTF CORRECTION METHOD             : NULL                           
REMARK   3                                                                      
REMARK   3 EM RECONSTRUCTION MAGNIFICATION CALIBRATION: NULL                    
REMARK   3                                                                      
REMARK   3 OTHER DETAILS: THE COORDINATES IN THIS ENTRY WERE GENERATED FROM     
REMARK   3  ELECTRON MICROSCOPY DATA. PROTEIN DATA BANK CONVENTIONS REQUIRE     
REMARK   3  THAT CRYST1 AND SCALE RECORDS BE INCLUDED, BUT THE VALUES ON        
REMARK   3  THESE RECORDS ARE MEANINGLESS. THE MRNA HAS BEEN MODELED USING      
REMARK   3  MANIP (MASSIRE AND WESTHOF).                                        
REMARK   4                                                                      
REMARK   4 2VAZ COMPLIES WITH FORMAT V. 3.30, 13-JUL-11                         
REMARK 100                                                                      
REMARK 100 THIS ENTRY HAS BEEN PROCESSED BY PDBE.                               
REMARK 100 THE DEPOSITION ID IS D_1290033682.                                   
REMARK 245                                                                      
REMARK 245 EXPERIMENTAL DETAILS                                                 
REMARK 245   RECONSTRUCTION METHOD          : SINGLE PARTICLE                   
REMARK 245   SPECIMEN TYPE                  : VITREOUS ICE (CRYO EM)            
REMARK 245                                                                      
REMARK 245 ELECTRON MICROSCOPE SAMPLE                                           
REMARK 245   SAMPLE TYPE                    : PARTICLE                          
REMARK 245   PARTICLE TYPE                  : POINT                             
REMARK 245   NAME OF SAMPLE                 : 70S PRE-INITIATION COMPLEX        
REMARK 245                                    ENTRAPPED BY S15- RPSOMRNA        
REMARK 245   SAMPLE CONCENTRATION (MG ML-1) : 0.50                              
REMARK 245   SAMPLE SUPPORT DETAILS         : HOLEY CARBON                      
REMARK 245   SAMPLE VITRIFICATION DETAILS   : BLOT FOR 2 SECONDS BEFORE         
REMARK 245                                    PLUNGING IN LIQUID ETHANE USING   
REMARK 245                                    A HOME-MADE CRYO- PLUNGER         
REMARK 245   SAMPLE BUFFER                  : 20 MM TRIS-HCL PH 7.5, 60 MM      
REMARK 245                                    KCL, 1MM DTT, 7.5 MM MGCL2        
REMARK 245   PH                             : 7.50                              
REMARK 245   SAMPLE DETAILS                 : NULL                              
REMARK 245                                                                      
REMARK 245 DATA ACQUISITION                                                     
REMARK 245   DATE OF EXPERIMENT                : 05-JAN-05                      
REMARK 245   NUMBER OF MICROGRAPHS-IMAGES      : NULL                           
REMARK 245   TEMPERATURE (KELVIN)              : 77.00                          
REMARK 245   MICROSCOPE MODEL                  : FEI TECNAI F20                 
REMARK 245   DETECTOR TYPE                     : KODAK SO-163 FILM              
REMARK 245   MINIMUM DEFOCUS (NM)              : 800.00                         
REMARK 245   MAXIMUM DEFOCUS (NM)              : 3500.00                        
REMARK 245   MINIMUM TILT ANGLE (DEGREES)      : NULL                           
REMARK 245   MAXIMUM TILT ANGLE (DEGREES)      : NULL                           
REMARK 245   NOMINAL CS                        : 2.00                           
REMARK 245   IMAGING MODE                      : BRIGHT FIELD                   
REMARK 245   ELECTRON DOSE (ELECTRONS NM**-2)  : 20.00                          
REMARK 245   ILLUMINATION MODE                 : FLOOD BEAM                     
REMARK 245   NOMINAL MAGNIFICATION             : 50000                          
REMARK 245   CALIBRATED MAGNIFICATION          : 51484                          
REMARK 245   SOURCE                            : FIELD EMISSION GUN             
REMARK 245   ACCELERATION VOLTAGE (KV)         : 200                            
REMARK 245   IMAGING DETAILS                   : NULL                           
REMARK 247                                                                      
REMARK 247 ELECTRON MICROSCOPY                                                  
REMARK 247  THE COORDINATES IN THIS ENTRY WERE GENERATED FROM ELECTRON          
REMARK 247  MICROSCOPY DATA. PROTEIN DATA BANK CONVENTIONS REQUIRE              
REMARK 247  THAT CRYST1 AND SCALE RECORDS BE INCLUDED, BUT THE VALUES           
REMARK 247  ON THESE RECORDS ARE MEANINGLESS EXCEPT FOR THE CALCULATION         
REMARK 247  OF THE STRUCTURE FACTORS.                                           
REMARK 300                                                                      
REMARK 300 BIOMOLECULE: 1                                                       
REMARK 300 SEE REMARK 350 FOR THE AUTHOR PROVIDED AND/OR PROGRAM                
REMARK 300 GENERATED ASSEMBLY INFORMATION FOR THE STRUCTURE IN                  
REMARK 300 THIS ENTRY. THE REMARK MAY ALSO PROVIDE INFORMATION ON               
REMARK 300 BURIED SURFACE AREA.                                                 
REMARK 350                                                                      
REMARK 350 COORDINATES FOR A COMPLETE MULTIMER REPRESENTING THE KNOWN           
REMARK 350 BIOLOGICALLY SIGNIFICANT OLIGOMERIZATION STATE OF THE                
REMARK 350 MOLECULE CAN BE GENERATED BY APPLYING BIOMT TRANSFORMATIONS          
REMARK 350 GIVEN BELOW.  BOTH NON-CRYSTALLOGRAPHIC AND                          
REMARK 350 CRYSTALLOGRAPHIC OPERATIONS ARE GIVEN.                               
REMARK 350                                                                      
REMARK 350 BIOMOLECULE: 1                                                       
REMARK 350 AUTHOR DETERMINED BIOLOGICAL UNIT: DIMERIC                           
REMARK 350 SOFTWARE DETERMINED QUATERNARY STRUCTURE: DIMERIC                    
REMARK 350 SOFTWARE USED: PQS                                                   
REMARK 350 APPLY THE FOLLOWING TO CHAINS: A, F                                  
REMARK 350   BIOMT1   1  1.000000  0.000000  0.000000        0.00000            
REMARK 350   BIOMT2   1  0.000000  1.000000  0.000000        0.00000            
REMARK 350   BIOMT3   1  0.000000  0.000000  1.000000        0.00000            
REMARK 465                                                                      
REMARK 465 MISSING RESIDUES                                                     
REMARK 465 THE FOLLOWING RESIDUES WERE NOT LOCATED IN THE                       
REMARK 465 EXPERIMENT. (M=MODEL NUMBER; RES=RESIDUE NAME; C=CHAIN               
REMARK 465 IDENTIFIER; SSSEQ=SEQUENCE NUMBER; I=INSERTION CODE.)                
REMARK 465                                                                      
REMARK 465   M RES C SSSEQI                                                     
REMARK 465       G A   -15                                                      
REMARK 465       G A   -14                                                      
REMARK 465       G A   -13                                                      
REMARK 465       C A   -12                                                      
REMARK 465       G A   -11                                                      
REMARK 465       A A   -10                                                      
REMARK 465       A A    -9                                                      
REMARK 465       U A    -8                                                      
REMARK 465       U A    -7                                                      
REMARK 465       C A    -6                                                      
REMARK 465       G A    -5                                                      
REMARK 465       A A    -4                                                      
REMARK 465       G A    -3                                                      
REMARK 465       C A    -2                                                      
REMARK 465       U A    -1                                                      
REMARK 465       C A     0                                                      
REMARK 465       G A     1                                                      
REMARK 465       G A     2                                                      
REMARK 465       U A     3                                                      
REMARK 465       A A     4                                                      
REMARK 465       C A     5                                                      
REMARK 465       C A     6                                                      
REMARK 465       C A     7                                                      
REMARK 465       A A     8                                                      
REMARK 465       C A    76                                                      
REMARK 465       U A    77                                                      
REMARK 465       U A    78                                                      
REMARK 465       U A    79                                                      
REMARK 465       C A    80                                                      
REMARK 465       A A    81                                                      
REMARK 465       U A    82                                                      
REMARK 465       U A    83                                                      
REMARK 465       C A    84                                                      
REMARK 465       U A    85                                                      
REMARK 465       A A    86                                                      
REMARK 465       U A    87                                                      
REMARK 465       A A    88                                                      
REMARK 465       U A    89                                                      
REMARK 465       A A    90                                                      
REMARK 465       C A    91                                                      
REMARK 465       U A   101                                                      
REMARK 465       A A   102                                                      
REMARK 465       A A   103                                                      
REMARK 465       A A   104                                                      
REMARK 465       A A   105                                                      
REMARK 465       U A   106                                                      
REMARK 465       G A   107                                                      
REMARK 465       U A   108                                                      
REMARK 465       G A   115                                                      
REMARK 465       U A   116                                                      
REMARK 465       A A   117                                                      
REMARK 465       C A   118                                                      
REMARK 465       U A   119                                                      
REMARK 465       G A   120                                                      
REMARK 465       A A   121                                                      
REMARK 465       A A   122                                                      
REMARK 465       G A   123                                                      
REMARK 465       C A   124                                                      
REMARK 465       A A   125                                                      
REMARK 465       A A   126                                                      
REMARK 465       C A   127                                                      
REMARK 465       A A   128                                                      
REMARK 465       G A   129                                                      
REMARK 465       C A   130                                                      
REMARK 465       U A   131                                                      
REMARK 465       A A   132                                                      
REMARK 465       A A   133                                                      
REMARK 465       A A   134                                                      
REMARK 465       A A   135                                                      
REMARK 465       U A   136                                                      
REMARK 465       C A   137                                                      
REMARK 465       G A   138                                                      
REMARK 465       U A   139                                                      
REMARK 465       U A   140                                                      
REMARK 465       U A   141                                                      
REMARK 465       C A   142                                                      
REMARK 465       U A   143                                                      
REMARK 465       G A   144                                                      
REMARK 465       A A   145                                                      
REMARK 465       G A   146                                                      
REMARK 465       U A   147                                                      
REMARK 465       U A   148                                                      
REMARK 465       U A   149                                                      
REMARK 465       G A   150                                                      
REMARK 465       G A   151                                                      
REMARK 465       U A   152                                                      
REMARK 465       C A   153                                                      
REMARK 465       G A   154                                                      
REMARK 465       A A   155                                                      
REMARK 465       C A   156                                                      
REMARK 465       C A   157                                                      
REMARK 465       U A   158                                                      
REMARK 465       G A   159                                                      
REMARK 465       C A   160                                                      
REMARK 465       A A   161                                                      
REMARK 465       G A   162                                                      
REMARK 465       G A   163                                                      
REMARK 465       C A   164                                                      
REMARK 465       A A   165                                                      
REMARK 465       U A   166                                                      
REMARK 465       G A   167                                                      
REMARK 465       C A   168                                                      
REMARK 465       A A   169                                                      
REMARK 465     MET F   100                                                      
REMARK 465     ARG F   187                                                      
REMARK 465     ARG F   188                                                      
REMARK 900                                                                      
REMARK 900 RELATED ENTRIES                                                      
REMARK 900 RELATED ID: EMD-1391   RELATED DB: EMDB                              
REMARK 900 STRUCTURED MRNAS REGULATE TRANSLATION INITIATION BY BINDING TO A     
REMARK 900 DEDICATED SITE ON THE RIBOSOME.                                      
REMARK 900 RELATED ID: 1P6G   RELATED DB: PDB                                   
REMARK 900 REAL SPACE REFINED COORDINATES OF THE 30S SUBUNIT FITTEDINTO THE     
REMARK 900 LOW RESOLUTION CRYO- EM MAP OF THE EF-G.GTP STATE OF E. COLI 70S     
REMARK 900 RIBOSOME                                                             
REMARK 900 RELATED ID: 1P87   RELATED DB: PDB                                   
REMARK 900 REAL SPACE REFINED COORDINATES OF THE 30S SUBUNIT FITTEDINTO THE     
REMARK 900 LOW RESOLUTION CRYO- EM MAP OF THE INITIATION-LIKESTATE OF E. COLI   
REMARK 900 70S RIBOSOME                                                         
REMARK 900 RELATED ID: 2AVY   RELATED DB: PDB                                   
REMARK 900 CRYSTAL STRUCTURE OF THE BACTERIAL RIBOSOME FROMESCHERICHIA COLI AT  
REMARK 900 3.5 A RESOLUTION. THIS FILE CONTAINS THE 30S SUBUNIT OF ONE 70S      
REMARK 900 RIBOSOME. THE ENTIRE CRYSTALSTRUCTURE CONTAINS TWO 70S RIBOSOMES     
REMARK 900 AND IS DESCRIBED INREMARK 400.                                       
REMARK 900 RELATED ID: 2AW7   RELATED DB: PDB                                   
REMARK 900 CRYSTAL STRUCTURE OF THE BACTERIAL RIBOSOME FROMESCHERICHIA COLI AT  
REMARK 900 3.5 A RESOLUTION. THIS FILE CONTAINS THE 30S SUBUNIT OF THE SECOND   
REMARK 900 70S RIBOSOME. THE ENTIRECRYSTAL STRUCTURE CONTAINS TWO 70S           
REMARK 900 RIBOSOMES AND ISDESCRIBED IN REMARK 400.                             
DBREF  2VAZ A  -15   169  PDB    2VAZ     2VAZ           -15    169             
DBREF  2VAZ F  100   100  PDB    2VAZ     2VAZ           100    100             
DBREF  2VAZ F  101   188  UNP    P0ADZ4   RS15_ECOLI       1     88             
SEQRES   1 A  185    G   G   G   C   G   A   A   U   U   C   G   A   G          
SEQRES   2 A  185    C   U   C   G   G   U   A   C   C   C   A   A   C          
SEQRES   3 A  185    G   U   C   G   C   G   U   A   A   A   U   U   G          
SEQRES   4 A  185    U   U   U   A   A   C   A   C   U   U   U   G   C          
SEQRES   5 A  185    G   U   A   A   C   G   U   A   C   A   C   U   G          
SEQRES   6 A  185    G   G   A   U   C   G   C   U   G   A   A   U   U          
SEQRES   7 A  185    A   G   A   G   A   U   C   G   G   C   G   U   C          
SEQRES   8 A  185    C   U   U   U   C   A   U   U   C   U   A   U   A          
SEQRES   9 A  185    U   A   C   U   A   A   G   G   A   G   G   U   U          
SEQRES  10 A  185    A   A   A   A   U   G   U   C   U   C   U   A   A          
SEQRES  11 A  185    G   U   A   C   U   G   A   A   G   C   A   A   C          
SEQRES  12 A  185    A   G   C   U   A   A   A   A   U   C   G   U   U          
SEQRES  13 A  185    U   C   U   G   A   G   U   U   U   G   G   U   C          
SEQRES  14 A  185    G   A   C   C   U   G   C   A   G   G   C   A   U          
SEQRES  15 A  185    G   C   A                                                  
SEQRES   1 F   89  MET SER LEU SER THR GLU ALA THR ALA LYS ILE VAL SER          
SEQRES   2 F   89  GLU PHE GLY ARG ASP ALA ASN ASP THR GLY SER THR GLU          
SEQRES   3 F   89  VAL GLN VAL ALA LEU LEU THR ALA GLN ILE ASN HIS LEU          
SEQRES   4 F   89  GLN GLY HIS PHE ALA GLU HIS LYS LYS ASP HIS HIS SER          
SEQRES   5 F   89  ARG ARG GLY LEU LEU ARG MET VAL SER GLN ARG ARG LYS          
SEQRES   6 F   89  LEU LEU ASP TYR LEU LYS ARG LYS ASP VAL ALA ARG TYR          
SEQRES   7 F   89  THR GLN LEU ILE GLU ARG LEU GLY LEU ARG ARG                  
CRYST1    1.000    1.000    1.000  90.00  90.00  90.00 P 1           1          
ORIGX1      1.000000  0.000000  0.000000        0.00000                         
ORIGX2      0.000000  1.000000  0.000000        0.00000                         
ORIGX3      0.000000  0.000000  1.000000        0.00000                         
SCALE1      1.000000  0.000000  0.000000        0.00000                         
SCALE2      0.000000  1.000000  0.000000        0.00000                         
SCALE3      0.000000  0.000000  1.000000        0.00000                         
ATOM      1  P     A A   9     -73.739 -93.192  14.845  1.00  1.00           P  
ATOM      2  P     C A  10     -68.586 -92.653  17.790  1.00  1.00           P  
ATOM      3  P     G A  11     -63.937 -90.604  20.040  1.00  1.00           P  
ATOM      4  P     U A  12     -61.019 -86.288  21.592  1.00  1.00           P  
ATOM      5  P     C A  13     -60.390 -81.187  23.379  1.00  1.00           P  
ATOM      6  P     G A  14     -62.639 -76.638  25.373  1.00  1.00           P  
ATOM      7  P     C A  15     -66.696 -74.009  27.775  1.00  1.00           P  
ATOM      8  P     G A  16     -70.893 -73.731  31.074  1.00  1.00           P  
ATOM      9  P     U A  17     -74.278 -76.028  34.714  1.00  1.00           P  
ATOM     10  P     A A  18     -75.412 -79.543  38.595  1.00  1.00           P  
ATOM     11  P     A A  19     -73.903 -83.380  42.222  1.00  1.00           P  
ATOM     12  P     A A  20     -70.220 -85.826  45.304  1.00  1.00           P  
ATOM     13  P     U A  21     -65.536 -86.015  47.968  1.00  1.00           P  
ATOM     14  P     U A  22     -60.655 -83.757  49.878  1.00  1.00           P  
ATOM     15  P     G A  23     -57.096 -79.155  50.363  1.00  1.00           P  
ATOM     16  P     U A  24     -56.655 -74.205  51.573  1.00  1.00           P  
ATOM     17  P     U A  25     -57.063 -71.102  55.340  1.00  1.00           P  
ATOM     18  P     U A  26     -61.986 -73.446  56.345  1.00  1.00           P  
ATOM     19  P     A A  27     -66.537 -74.654  54.411  1.00  1.00           P  
ATOM     20  P     A A  28     -69.541 -74.583  49.910  1.00  1.00           P  
ATOM     21  P     C A  29     -69.569 -72.741  44.517  1.00  1.00           P  
ATOM     22  P     A A  30     -67.167 -71.620  39.820  1.00  1.00           P  
ATOM     23  P     C A  31     -62.796 -73.262  37.367  1.00  1.00           P  
ATOM     24  P     U A  32     -59.890 -76.907  35.443  1.00  1.00           P  
ATOM     25  P     U A  33     -59.341 -81.808  33.646  1.00  1.00           P  
ATOM     26  P     U A  34     -61.301 -86.520  31.990  1.00  1.00           P  
ATOM     27  P     G A  35     -65.242 -89.712  30.147  1.00  1.00           P  
ATOM     28  P     C A  36     -70.191 -90.495  28.077  1.00  1.00           P  
ATOM     29  P     G A  37     -74.428 -89.004  25.175  1.00  1.00           P  
ATOM     30  P     U A  38     -76.942 -85.501  21.813  1.00  1.00           P  
ATOM     31  P     A A  39     -77.041 -81.795  18.001  1.00  1.00           P  
ATOM     32  P     A A  40     -74.538 -78.499  14.248  1.00  1.00           P  
ATOM     33  P     C A  41     -70.860 -77.387  10.794  1.00  1.00           P  
ATOM     34  P     G A  42     -66.607 -78.656   7.551  1.00  1.00           P  
ATOM     35  P     U A  43     -61.307 -81.728   5.790  1.00  1.00           P  
ATOM     36  P     A A  44     -56.168 -84.853   6.910  1.00  0.00           P  
ATOM     37  P     C A  45     -52.261 -89.829   7.966  1.00  0.00           P  
ATOM     38  P     A A  46     -48.914 -93.686  11.082  1.00  0.00           P  
ATOM     39  P     C A  47     -48.215 -97.425  14.361  1.00  0.00           P  
ATOM     40  P     U A  48     -50.335-100.589  17.317  1.00  0.00           P  
ATOM     41  P     G A  49     -54.308-102.295  20.610  1.00  1.00           P  
ATOM     42  P     G A  50     -56.602-107.214  22.589  1.00  1.00           P  
ATOM     43  P     G A  51     -60.076-111.549  21.446  1.00  1.00           P  
ATOM     44  P     A A  52     -63.109-114.757  18.045  1.00  1.00           P  
ATOM     45  P     U A  53     -63.844-116.469  13.378  1.00  1.00           P  
ATOM     46  P     C A  54     -66.223-116.239   7.316  1.00  1.00           P  
ATOM     47  P     G A  55     -65.997-114.532   1.234  1.00  1.00           P  
ATOM     48  P     C A  56     -63.667-112.953  -3.423  1.00  1.00           P  
ATOM     49  P     U A  57     -59.022-112.243  -6.599  1.00  1.00           P  
ATOM     50  P     G A  58     -53.320-113.541  -7.426  1.00  1.00           P  
ATOM     51  P     A A  59     -49.141-116.433  -7.957  1.00  1.00           P  
ATOM     52  P     A A  60     -45.484-120.514  -7.633  1.00  1.00           P  
ATOM     53  P     U A  61     -42.273-124.499 -12.575  1.00  1.00           P  
ATOM     54  P     U A  62     -46.810-123.836 -14.411  1.00  1.00           P  
ATOM     55  P     A A  63     -51.775-121.518 -14.603  1.00  1.00           P  
ATOM     56  P     G A  64     -56.914-120.810 -12.667  1.00  1.00           P  
ATOM     57  P     A A  65     -60.751-121.748  -8.703  1.00  1.00           P  
ATOM     58  P     G A  66     -62.240-123.271  -3.507  1.00  1.00           P  
ATOM     59  P     A A  67     -60.969-124.232   1.902  1.00  1.00           P  
ATOM     60  P     U A  68     -57.865-123.580   6.171  1.00  1.00           P  
ATOM     61  P     C A  69     -53.278-120.532   8.127  1.00  1.00           P  
ATOM     62  P     G A  70     -50.705-116.042   9.059  1.00  1.00           P  
ATOM     63  P     G A  71     -50.110-110.647   8.215  1.00  1.00           P  
ATOM     64  P     C A  72     -52.476-105.662   7.084  1.00  1.00           P  
ATOM     65  P     G A  73     -56.517-101.882   6.640  1.00  1.00           P  
ATOM     66  P     U A  74     -61.632-100.165   7.584  1.00  1.00           P  
ATOM     67  P     C A  75     -66.801-100.166   9.593  1.00  1.00           P  
ATOM     68  P     U A  92     -51.549 -88.814  24.621  1.00  0.00           P  
ATOM     69  P     A A  93     -54.079 -84.065  23.584  1.00  0.00           P  
ATOM     70  P     A A  94     -53.577 -78.184  23.375  1.00  0.00           P  
ATOM     71  P     G A  95     -50.811 -73.035  23.730  1.00  0.00           P  
ATOM     72  P     G A  96     -45.281 -71.470  22.240  1.00  0.00           P  
ATOM     73  P     A A  97     -40.980 -72.114  18.245  1.00  0.00           P  
ATOM     74  P     G A  98     -38.863 -74.465  13.497  1.00  0.00           P  
ATOM     75  P     G A  99     -39.487 -77.893   9.076  1.00  0.00           P  
ATOM     76  P     U A 100     -42.332 -81.844   4.152  1.00  0.00           P  
ATOM     77  P     C A 109     -45.502-129.122  -3.186  1.00  1.00           P  
ATOM     78  P     U A 110     -48.999-133.894  -3.938  1.00  1.00           P  
ATOM     79  P     C A 111     -53.209-136.742  -6.305  1.00  1.00           P  
ATOM     80  P     U A 112     -56.676-137.712 -10.509  1.00  1.00           P  
ATOM     81  P     A A 113     -58.264-136.891 -15.871  1.00  1.00           P  
ATOM     82  P     A A 114     -57.244-135.192 -21.376  1.00  1.00           P  
TER      83        A A 114                                                      
ATOM     84  CA  SER F 101     -62.376-115.114 -12.342  1.00  0.90           C  
ATOM     85  CA  LEU F 102     -61.032-114.207 -15.774  1.00 99.71           C  
ATOM     86  CA  SER F 103     -63.089-116.901 -17.521  1.00  0.08           C  
ATOM     87  CA  THR F 104     -62.612-118.733 -20.739  1.00 96.10           C  
ATOM     88  CA  GLU F 105     -64.437-116.348 -23.026  1.00  0.18           C  
ATOM     89  CA  ALA F 106     -61.131-114.550 -22.553  1.00 93.38           C  
ATOM     90  CA  THR F 107     -58.384-116.850 -21.479  1.00 74.20           C  
ATOM     91  CA  ALA F 108     -59.393-119.378 -24.104  1.00 77.66           C  
ATOM     92  CA  LYS F 109     -60.024-116.601 -26.574  1.00 74.45           C  
ATOM     93  CA  ILE F 110     -56.444-115.401 -26.387  1.00 63.59           C  
ATOM     94  CA  VAL F 111     -54.985-118.927 -26.520  1.00 63.60           C  
ATOM     95  CA  SER F 112     -57.031-119.771 -29.582  1.00 71.86           C  
ATOM     96  CA  GLU F 113     -55.375-116.758 -31.171  1.00 77.54           C  
ATOM     97  CA  PHE F 114     -51.711-117.099 -30.259  1.00 70.74           C  
ATOM     98  CA  GLY F 115     -51.650-120.900 -30.098  1.00 65.36           C  
ATOM     99  CA  ARG F 116     -49.270-122.580 -32.543  1.00 60.52           C  
ATOM    100  CA  ASP F 117     -51.128-126.135 -33.222  1.00 57.55           C  
ATOM    101  CA  ALA F 118     -53.544-128.957 -31.950  1.00 57.46           C  
ATOM    102  CA  ASN F 119     -51.758-129.866 -28.739  1.00 45.45           C  
ATOM    103  CA  ASP F 120     -49.426-126.968 -28.140  1.00 47.11           C  
ATOM    104  CA  THR F 121     -49.411-126.243 -24.396  1.00 30.90           C  
ATOM    105  CA  GLY F 122     -46.138-124.405 -23.830  1.00 33.56           C  
ATOM    106  CA  SER F 123     -45.022-122.703 -27.042  1.00 35.92           C  
ATOM    107  CA  THR F 124     -43.269-119.569 -25.650  1.00 40.52           C  
ATOM    108  CA  GLU F 125     -45.940-117.518 -27.241  1.00 43.37           C  
ATOM    109  CA  VAL F 126     -48.711-118.983 -25.195  1.00 44.99           C  
ATOM    110  CA  GLN F 127     -46.402-118.830 -22.204  1.00 46.30           C  
ATOM    111  CA  VAL F 128     -45.751-115.129 -22.670  1.00 46.62           C  
ATOM    112  CA  ALA F 129     -49.286-114.152 -23.603  1.00 50.24           C  
ATOM    113  CA  LEU F 130     -51.049-115.659 -20.635  1.00 50.88           C  
ATOM    114  CA  LEU F 131     -48.092-114.345 -18.652  1.00 52.34           C  
ATOM    115  CA  THR F 132     -48.975-110.829 -19.767  1.00 58.44           C  
ATOM    116  CA  ALA F 133     -52.581-111.723 -18.985  1.00 61.71           C  
ATOM    117  CA  GLN F 134     -52.094-112.112 -15.249  1.00 70.49           C  
ATOM    118  CA  ILE F 135     -49.544-109.306 -15.239  1.00 63.90           C  
ATOM    119  CA  ASN F 136     -52.342-106.860 -16.068  1.00 62.17           C  
ATOM    120  CA  HIS F 137     -54.964-108.762 -14.076  1.00 60.52           C  
ATOM    121  CA  LEU F 138     -52.776-107.900 -11.075  1.00 57.84           C  
ATOM    122  CA  GLN F 139     -51.495-104.575 -12.126  1.00 68.33           C  
ATOM    123  CA  GLY F 140     -55.059-103.539 -11.846  1.00 66.64           C  
ATOM    124  CA  HIS F 141     -55.363-105.302  -8.484  1.00 63.41           C  
ATOM    125  CA  PHE F 142     -52.409-103.500  -6.920  1.00 64.79           C  
ATOM    126  CA  ALA F 143     -54.263-100.255  -7.788  1.00 70.83           C  
ATOM    127  CA  GLU F 144     -57.317-100.809  -5.545  1.00 69.10           C  
ATOM    128  CA  HIS F 145     -54.986-102.090  -2.783  1.00 76.62           C  
ATOM    129  CA  LYS F 146     -51.924 -99.821  -2.700  1.00 83.30           C  
ATOM    130  CA  LYS F 147     -50.973-101.215   0.721  1.00 86.82           C  
ATOM    131  CA  ASP F 148     -50.618-104.809  -0.499  1.00 89.16           C  
ATOM    132  CA  HIS F 149     -47.194-104.839  -2.040  1.00 87.30           C  
ATOM    133  CA  HIS F 150     -46.563-108.332  -0.954  1.00 75.88           C  
ATOM    134  CA  SER F 151     -48.212-109.042  -4.134  1.00 59.14           C  
ATOM    135  CA  ARG F 152     -45.884-106.657  -5.841  1.00 57.10           C  
ATOM    136  CA  ARG F 153     -43.056-109.157  -5.281  1.00 49.49           C  
ATOM    137  CA  GLY F 154     -44.717-111.406  -7.821  1.00 46.62           C  
ATOM    138  CA  LEU F 155     -45.555-108.574 -10.175  1.00 43.44           C  
ATOM    139  CA  LEU F 156     -41.787-108.191 -10.496  1.00 51.16           C  
ATOM    140  CA  ARG F 157     -41.078-111.892 -10.894  1.00 51.17           C  
ATOM    141  CA  MET F 158     -43.814-112.390 -13.493  1.00 43.28           C  
ATOM    142  CA  VAL F 159     -42.515-109.334 -15.408  1.00 41.35           C  
ATOM    143  CA  SER F 160     -38.965-110.679 -15.410  1.00 46.24           C  
ATOM    144  CA  GLN F 161     -39.987-114.149 -16.547  1.00 50.12           C  
ATOM    145  CA  ARG F 162     -42.020-112.497 -19.320  1.00 43.91           C  
ATOM    146  CA  ARG F 163     -38.990-110.615 -20.590  1.00 53.32           C  
ATOM    147  CA  LYS F 164     -36.718-113.699 -20.399  1.00 47.98           C  
ATOM    148  CA  LEU F 165     -39.000-115.787 -22.631  1.00 46.35           C  
ATOM    149  CA  LEU F 166     -39.384-112.886 -25.081  1.00 47.84           C  
ATOM    150  CA  ASP F 167     -35.631-112.507 -25.282  1.00 48.34           C  
ATOM    151  CA  TYR F 168     -35.303-116.241 -25.820  1.00 48.01           C  
ATOM    152  CA  LEU F 169     -37.885-116.154 -28.591  1.00 49.59           C  
ATOM    153  CA  LYS F 170     -36.105-113.124 -30.047  1.00 66.87           C  
ATOM    154  CA  ARG F 171     -32.876-115.055 -30.656  1.00 74.14           C  
ATOM    155  CA  LYS F 172     -34.198-118.380 -31.912  1.00 79.06           C  
ATOM    156  CA  ASP F 173     -36.948-116.813 -34.061  1.00 86.09           C  
ATOM    157  CA  VAL F 174     -36.974-113.067 -34.940  1.00 87.12           C  
ATOM    158  CA  ALA F 175     -39.929-113.128 -37.348  1.00 92.49           C  
ATOM    159  CA  ARG F 176     -42.184-114.690 -34.714  1.00 83.29           C  
ATOM    160  CA  TYR F 177     -40.882-112.215 -32.145  1.00 82.08           C  
ATOM    161  CA  THR F 178     -42.152-109.289 -34.173  1.00 93.27           C  
ATOM    162  CA  GLN F 179     -45.482-110.990 -34.598  1.00 92.37           C  
ATOM    163  CA  LEU F 180     -46.000-111.722 -30.920  1.00 80.47           C  
ATOM    164  CA  ILE F 181     -44.824-108.326 -29.665  1.00 88.74           C  
ATOM    165  CA  GLU F 182     -47.878-107.148 -31.563  1.00 95.03           C  
ATOM    166  CA  ARG F 183     -51.446-107.961 -30.612  1.00 93.44           C  
ATOM    167  CA  LEU F 184     -50.017-106.932 -27.311  1.00 94.57           C  
ATOM    168  CA  GLY F 185     -47.381-104.335 -28.034  1.00 98.39           C  
ATOM    169  CA  LEU F 186     -43.885-104.023 -26.544  1.00  0.34           C  
TER     170      LEU F 186                                                      
MASTER      239    0    0    0    0    0    0    6  168    2    0   22          
END                                                                             
