5H9F

Crystal structure of E. coli Cascade bound to a PAM-containing dsDNA target at 2.45 angstrom resolution.

Summary for 5H9F

• Entry DOI: 10.2210/pdb5h9f/pdb
• Related: 5H9E
• Descriptor: CRISPR system Cascade subunit CasA, CRISPR system Cascade subunit CasB, CRISPR system Cascade subunit CasC, ... (10 entities in total)
• Functional Keywords: crispr cascade, immune system-rna complex, immune system/rna
• Biological source: Escherichia coli (strain K12); More
• Total number of polymer chains: 14
• Total formula weight: 426150.75

Authors

Hayes, R.P.,Xiao, Y.,Ding, F.,van Erp, P.B.G.,Rajashankar, K.,Bailey, S.,Wiedenheft, B.,Ke, A. (deposition date: 2015-12-28, release date: 2016-02-17, Last modification date: 2023-09-27)

• Primary citation: Hayes, R.P.,Xiao, Y.,Ding, F.,van Erp, P.B.,Rajashankar, K.,Bailey, S.,Wiedenheft, B.,Ke, A.; Structural basis for promiscuous PAM recognition in type I-E Cascade from E. coli.; Nature, 530:499-503, 2016; PubMed: 26863189; DOI: 10.1038/nature16995

Experimental method

X-RAY DIFFRACTION (2.45 Å)