5G1K

A triple mutant of DsbG engineered for denitrosylation

Summary for 5G1K

• Entry DOI: 10.2210/pdb5g1k/pdb
• Related: 5G1L
• Descriptor: THIOL DISULFIDE INTERCHANGE PROTEIN DSBG, SULFATE ION (3 entities in total)
• Functional Keywords: isomerase, s-(de)nitrosylation, trx family, cxxc motif, dsbg
• Biological source: ESCHERICHIA COLI
• Cellular location: Periplasm: P77202
• Total number of polymer chains: 2
• Total formula weight: 57360.39

Authors

Tamu Dufe, V.,Van Molle, I.,Lafaye, C.,Wahni, K.,Boudier, A.,Leroy, P.,Collet, J.F.,Messens, J. (deposition date: 2016-03-28, release date: 2016-05-25, Last modification date: 2024-01-10)

• Primary citation: Lafaye, C.,Van Molle, I.,Tamu Dufe, V.,Wahni, K.,Boudier, A.,Leroy, P.,Collet, J.,Messens, J.; Sulfur Denitrosylation by an Engineered Trx-Like Dsbg Enzyme Identifies Nucleophilic Cysteine Hydrogen Bonds as Key Functional Determinant.; J.Biol.Chem., 291:15020-, 2016; PubMed: 27226614; DOI: 10.1074/JBC.M116.729426

Experimental method

X-RAY DIFFRACTION (1.96 Å)