5FW1

Crystal structure of SpyCas9 variant VQR bound to sgRNA and TGAG PAM target DNA

Summary for 5FW1

Entry DOI	10.2210/pdb5fw1/pdb
Related	5FW2 5FW3
Descriptor	SGRNA, CRISPR-ASSOCIATED ENDONUCLEASE CAS9/CSN1, TARGET DNA STRAND, ... (7 entities in total)
Functional Keywords	hydrolase-dna complex, crispr, cas9, endonuclease, pam, genome editing, rnp, protein-rna complex, hydrolase/dna
Biological source	STREPTOCOCCUS PYOGENES More
Total number of polymer chains	4
Total formula weight	198509.97
Authors	Anders, C.,Bargsten, K.,Jinek, M. (deposition date: 2016-02-11, release date: 2016-04-20, Last modification date: 2024-01-10)
Primary citation	Anders, C.,Bargsten, K.,Jinek, M. Structural Plasticity of Pam Recognition by Engineered Variants of the RNA-Guided Endonuclease Cas9. Mol.Cell, 61:895-, 2016 Cited by PubMed: 26990992 DOI: 10.1016/J.MOLCEL.2016.02.020 PDB entries with the same primary citation
Experimental method	X-RAY DIFFRACTION (2.499 Å)

Structure validation