5E1Q

Mutant (D415G) GH97 alpha-galactosidase in complex with Gal-Lac

Summary for 5E1Q

• Entry DOI: 10.2210/pdb5e1q/pdb
• Descriptor: Retaining alpha-galactosidase, alpha-D-galactopyranose-(1-1)-[beta-D-galactopyranose-(1-4)]beta-D-glucopyranose, CALCIUM ION, ... (5 entities in total)
• Functional Keywords: alpha-galactosidase, hydrolase
• Biological source: Bacteroides thetaiotaomicron (strain ATCC 29148 / DSM 2079 / NCTC 10582 / E50 / VPI-5482)
• Total number of polymer chains: 2
• Total formula weight: 150059.50

Authors

Matsunaga, K.,Yamashita, K.,Tagami, T.,Yao, M.,Okuyama, M.,Kimura, A. (deposition date: 2015-09-30, release date: 2016-10-05, Last modification date: 2023-11-08)

• Primary citation: Okuyama, M.,Matsunaga, K.,Watanabe, K.I.,Yamashita, K.,Tagami, T.,Kikuchi, A.,Ma, M.,Klahan, P.,Mori, H.,Yao, M.,Kimura, A.; Efficient synthesis of alpha-galactosyl oligosaccharides using a mutant Bacteroides thetaiotaomicron retaining alpha-galactosidase (BtGH97b).; FEBS J., 284:766-783, 2017; PubMed: 28103425; DOI: 10.1111/febs.14018

Experimental method

X-RAY DIFFRACTION (1.943 Å)