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5GW7

Crystal structure of the glycosynthase mutant E727A of Escherichia coli GH63 glycosidase in complex with glucose and lactose

Replaces:  5CA4
Summary for 5GW7
Entry DOI10.2210/pdb5gw7/pdb
Related3D3I 3W7S 3W7T 3W7U 3W7W 3W7X 5CA3
Related PRD IDPRD_900008
DescriptorGlucosidase YgjK, beta-D-galactopyranose-(1-4)-alpha-D-glucopyranose, CALCIUM ION, ... (6 entities in total)
Functional Keywordsglycoside hydrolase, gh63 alpha, alpha barrel, hydrolase
Biological sourceEscherichia coli K-12
Total number of polymer chains2
Total formula weight173067.23
Authors
Miyazaki, T.,Tonozuka, T. (deposition date: 2016-09-08, release date: 2016-10-12, Last modification date: 2023-11-08)
Primary citationMiyazaki, T.,Nishikawa, A.,Tonozuka, T.
Crystal structure of the enzyme-product complex reveals sugar ring distortion during catalysis by family 63 inverting alpha-glycosidase
J.Struct.Biol., 196:479-486, 2016
Cited by
PubMed: 27688023
DOI: 10.1016/j.jsb.2016.09.015
PDB entries with the same primary citation
Experimental method
X-RAY DIFFRACTION (2.2 Å)
Structure validation

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