5B2T
Crystal structure of the Streptococcus pyogenes Cas9 VRER variant in complex with sgRNA and target DNA (TGCG PAM)
Summary for 5B2T
Entry DOI | 10.2210/pdb5b2t/pdb |
Related | 5B2R 5B2S |
Descriptor | Guide RNA, CRISPR-associated endonuclease Cas9, Target DNA, ... (9 entities in total) |
Functional Keywords | crispr-cas9, genome engineering, hydrolase-rna-dna complex, hydrolase/rna/dna |
Biological source | Streptococcus pyogenes serotype M1 More |
Total number of polymer chains | 4 |
Total formula weight | 197056.21 |
Authors | Hirano, S.,Nishimasu, H.,Ishitani, R.,Nureki, O. (deposition date: 2016-02-02, release date: 2016-03-23, Last modification date: 2023-11-08) |
Primary citation | Hirano, S.,Nishimasu, H.,Ishitani, R.,Nureki, O. Structural Basis for the Altered PAM Specificities of Engineered CRISPR-Cas9 Mol.Cell, 61:886-894, 2016 Cited by PubMed: 26990991DOI: 10.1016/j.molcel.2016.02.018 PDB entries with the same primary citation |
Experimental method | X-RAY DIFFRACTION (2.2 Å) |
Structure validation
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