5U33

Crystal structure of AacC2c1-sgRNA-extended non-target DNA ternary complex

Summary for 5U33

Entry DOI	10.2210/pdb5u33/pdb
Related	5U30 5U31 5U34
Descriptor	CRISPR-associated endonuclease C2c1, sgRNA, Target DNA strand, ... (5 entities in total)
Functional Keywords	type v crispr-cas endonculease: c2c1: structure: binary complex with sgrna: ternary complex with added dna: ruvc catalytic pocket: sequence-specific pam recognition: genome editing tool, hydrolase-dna complex, hydrolase/dna
Biological source	Alicyclobacillus acidoterrestris (strain ATCC 49025 / DSM 3922 / CIP 106132 / NCIMB 13137 / GD3B) More
Total number of polymer chains	4
Total formula weight	184106.79
Authors	Yang, H.,Gao, P.,Rajashankar, K.R.,Patel, D.J. (deposition date: 2016-12-01, release date: 2017-01-25, Last modification date: 2023-10-04)
Primary citation	Yang, H.,Gao, P.,Rajashankar, K.R.,Patel, D.J. PAM-Dependent Target DNA Recognition and Cleavage by C2c1 CRISPR-Cas Endonuclease. Cell, 167:1814-1828.e12, 2016 Cited by PubMed: 27984729 DOI: 10.1016/j.cell.2016.11.053 PDB entries with the same primary citation
Experimental method	X-RAY DIFFRACTION (3.75 Å)

Structure validation