4W2I

Crystal structure of the Thermus thermophilus 70S ribosome in complex with negamycin, mRNA and three deacylated tRNAs in the A, P and E sites

This is a non-PDB format compatible entry.

Summary for 4W2I

• Entry DOI: 10.2210/pdb4w2i/pdb
• Descriptor: 16S Ribosomal RNA, 30S Ribosomal Protein S10, 30S Ribosomal Protein S11, ... (61 entities in total)
• Functional Keywords: negamycin, antibiotic, 70s ribosome, inhibition of translation, miscoding, translocation inhibitor, 30s subunit with negamycin, mrna, deacylated a-, p-, and e-site trnas of the 1st 70s ribosome in the asu, functional complex of bacterial 70s ribosome with antibiotic negamycin, 50s subunit of the 1st 70s ribosome in the asu, ribosome-antibiotic complex, ribosome/antibiotic
• Biological source: Escherichia coli; More
• Total number of polymer chains: 112
• Total formula weight: 4555287.71

Authors

Polikanov, Y.S.,Szal, T.,Jiang, F.,Gupta, P.,Matsuda, R.,Shiozuka, M.,Steitz, T.A.,Vazquez-Laslop, N.,Mankin, A.S. (deposition date: 2014-09-12, release date: 2014-10-15, Last modification date: 2023-12-27)

• Primary citation: Polikanov, Y.S.,Szal, T.,Jiang, F.,Gupta, P.,Matsuda, R.,Shiozuka, M.,Steitz, T.A.,Vazquez-Laslop, N.,Mankin, A.S.; Negamycin Interferes with Decoding and Translocation by Simultaneous Interaction with rRNA and tRNA.; Mol.Cell, 56:541-550, 2014; PubMed: 25306922; DOI: 10.1016/j.molcel.2014.09.021

Experimental method

X-RAY DIFFRACTION (2.7 Å)