4U2F

Crystal structure of dienelactone hydrolase B-1 variant (Q35H, F38L, Y64H, Q110L, C123S, Y137C, Y145C, N154D, E199G, S208G and G211D) at 1.80 A resolution

Summary for 4U2F

• Entry DOI: 10.2210/pdb4u2f/pdb
• Related: 4U2B 4U2C 4U2D 4U2E 4U2G
• Descriptor: Carboxymethylenebutenolidase, SULFATE ION (3 entities in total)
• Functional Keywords: hydrolase, a/b hydrolase fold
• Biological source: Pseudomonas sp.
• Total number of polymer chains: 1
• Total formula weight: 25278.57

Authors

Porter, J.L.,Collyer, C.A.,Ollis, D.L. (deposition date: 2014-07-16, release date: 2014-12-10, Last modification date: 2023-12-27)

• Primary citation: Porter, J.L.,Boon, P.L.,Murray, T.P.,Huber, T.,Collyer, C.A.,Ollis, D.L.; Directed evolution of new and improved enzyme functions using an evolutionary intermediate and multidirectional search.; Acs Chem.Biol., 10:611-621, 2015; PubMed: 25419863; DOI: 10.1021/cb500809f

Experimental method

X-RAY DIFFRACTION (1.8 Å)