4MDE

Structure of bacterial polynucleotide kinase product complex bound to GDP and DNA

Summary for 4MDE

• Entry DOI: 10.2210/pdb4mde/pdb
• Related: 4GP6 4GP7 4JST 4JSY 4JT2 4JT4 4MDF
• Descriptor: Metallophosphoesterase, DNA (5'-D(P*CP*CP*TP*GP*T)-3'), GUANOSINE-5'-DIPHOSPHATE, ... (5 entities in total)
• Functional Keywords: rna repair, p-loop phosphotransferase, transferase, hydrolase-dna complex, transferase-dna complex, transferase/dna
• Biological source: Clostridium thermocellum; More
• Total number of polymer chains: 4
• Total formula weight: 42851.66

Authors

Shuman, S.,Das, U.,Wang, L.K.,Smith, P.,Jacewicz, A. (deposition date: 2013-08-22, release date: 2013-11-06, Last modification date: 2024-02-28)

• Primary citation: Das, U.,Wang, L.K.,Smith, P.,Jacewicz, A.,Shuman, S.; Structures of bacterial polynucleotide kinase in a Michaelis complex with GTP*Mg2+ and 5'-OH oligonucleotide and a product complex with GDP*Mg2+ and 5'-PO4 oligonucleotide reveal a mechanism of general acid-base catalysis and the determinants of phosphoacceptor recognition.; Nucleic Acids Res., 42:1152-1161, 2014; PubMed: 24150947; DOI: 10.1093/nar/gkt936

Experimental method

X-RAY DIFFRACTION (1.8 Å)