4KPW

Crystal structure of His-tagged human thymidylate synthase R175A mutant

Summary for 4KPW

Entry DOI	10.2210/pdb4kpw/pdb
Related	3N5E 3N5G
Descriptor	Thymidylate synthase, SULFATE ION, 1,2-ETHANEDIOL, ... (4 entities in total)
Functional Keywords	interface hot spot, transferase, methyltransferase, nucleotide biosynthesis
Biological source	Homo sapiens (human)
Cellular location	Nucleus : P04818
Total number of polymer chains	1
Total formula weight	37831.17
Authors	Pozzi, C.,Mangani, S. (deposition date: 2013-05-14, release date: 2014-05-14, Last modification date: 2023-09-20)
Primary citation	Salo-Ahen, O.M.,Tochowicz, A.,Pozzi, C.,Cardinale, D.,Ferrari, S.,Boum, Y.,Mangani, S.,Stroud, R.M.,Saxena, P.,Myllykallio, H.,Costi, M.P.,Ponterini, G.,Wade, R.C. Hotspots in an obligate homodimeric anticancer target. Structural and functional effects of interfacial mutations in human thymidylate synthase. J.Med.Chem., 58:3572-3581, 2015 Cited by PubMed: 25798950 DOI: 10.1021/acs.jmedchem.5b00137 PDB entries with the same primary citation
Experimental method	X-RAY DIFFRACTION (2.03 Å)

Structure validation