4KI2

Crystallographic analysis of an RNA-polymerase sigma-subunit fragment complexed with -10 promoter element ssDNA

Replaces:  4GOR

Summary for 4KI2

• Entry DOI: 10.2210/pdb4ki2/pdb
• Related: 3UGO 3UGP
• Descriptor: DNA (5'-D(*TP*GP*TP*AP*CP*AP*AP*TP*GP*GP*G)-3'), RNA polymerase sigma factor, POTASSIUM ION (3 entities in total)
• Functional Keywords: ssdna, g-quartet, g-quadruplex, promoter recognition, promoter opening, transcription initiation, protein-dna binding, ssdna-binding, transcription-dna complex, transcription/dna
• Biological source: Thermus aquaticus; More
• Cellular location: Cytoplasm : Q9EZJ8
• Total number of polymer chains: 6
• Total formula weight: 69925.27

Authors

Feklistov, A.,Darst, S.A. (deposition date: 2013-05-01, release date: 2013-09-04, Last modification date: 2024-02-28)

• Primary citation: Feklistov, A.,Darst, S.A.; Crystallographic analysis of an RNA polymerase sigma-subunit fragment complexed with -10 promoter element ssDNA: quadruplex formation as a possible tool for engineering crystal contacts in protein-ssDNA complexes.; Acta Crystallogr.,Sect.F, 69:950-955, 2013; PubMed: 23989139; DOI: 10.1107/S1744309113020368

Experimental method

X-RAY DIFFRACTION (3.3 Å)