4II2

Crystal structure of Ubiquitin activating enzyme 1 (Uba1) in complex with the Ub E2 Ubc4, ubiquitin, and ATP/Mg

Summary for 4II2

Entry DOI	10.2210/pdb4ii2/pdb
Related	4II3
Descriptor	Ubiquitin-activating enzyme E1 1, Ubiquitin-60S ribosomal protein L40, Ubiquitin-conjugating enzyme E2 4, ... (9 entities in total)
Functional Keywords	ubiquitin, e1, e2, uba1, ubc4, conformational change, thioester, adenylation, thioester transfer (transthioesterification), atp-binding, rossmann-like fold, ubiquitin-like fold, ligase activity, atp/mg binding, ubiquitin e2 binding, ubiquitination, nucleus, ligase
Biological source	Schizosaccharomyces pombe (Fission yeast) More
Cellular location	Cytoplasm: O94609 Ubiquitin: Cytoplasm . 60S ribosomal protein L40: Cytoplasm : P0CH07
Total number of polymer chains	3
Total formula weight	141137.09
Authors	Olsen, S.K.,Lima, C.D. (deposition date: 2012-12-19, release date: 2013-02-13, Last modification date: 2023-09-20)
Primary citation	Olsen, S.K.,Lima, C.D. Structure of a ubiquitin E1-E2 complex: insights to E1-E2 thioester transfer. Mol.Cell, 49:884-896, 2013 Cited by PubMed: 23416107 DOI: 10.1016/j.molcel.2013.01.013 PDB entries with the same primary citation
Experimental method	X-RAY DIFFRACTION (2.2 Å)

Structure validation