4HST

Crystal structure of a double mutant of a class III engineered cephalosporin acylase

Summary for 4HST

Entry DOI	10.2210/pdb4hst/pdb
Related	4HSR
Descriptor	glutaryl-7-aminocephalosporanic acid acylase alpha chain, glutaryl-7-aminocephalosporanic acid acylase beta chain, 5,5-dihydroxy-L-norvaline, ... (4 entities in total)
Functional Keywords	protein engineering, substrate specificity, transition state analogue, n-terminal hydrolase, hydrolase
Biological source	Pseudomonas More
Total number of polymer chains	2
Total formula weight	84231.23
Authors	Vrielink, A.,Golden, E.,Patterson, R.,Tie, W.J.,Anandan, A.,Flematti, G.,Molla, G.,Rosini, E.,Pollegioni, L. (deposition date: 2012-10-30, release date: 2013-02-27, Last modification date: 2024-02-28)
Primary citation	Golden, E.,Paterson, R.,Tie, W.J.,Anandan, A.,Flematti, G.,Molla, G.,Rosini, E.,Pollegioni, L.,Vrielink, A. Structure of a class III engineered cephalosporin acylase: comparisons with class I acylase and implications for differences in substrate specificity and catalytic activity. Biochem.J., 451:217-226, 2013 Cited by PubMed: 23373797 DOI: 10.1042/BJ20121715 PDB entries with the same primary citation
Experimental method	X-RAY DIFFRACTION (1.571 Å)

Structure validation