4CXU

G4 mutant of PAS, arylsulfatase from Pseudomonas Aeruginosa, in complex with 3-Br-Phenolphenylphosphonate

Summary for 4CXU

• Entry DOI: 10.2210/pdb4cxu/pdb
• Related: 4CXK 4CXS
• Descriptor: ARYLSULFATASE, CALCIUM ION, 3-bromophenyl hydrogen (S)-phenylphosphonate, ... (4 entities in total)
• Functional Keywords: hydrolase, catalytic promiscuity, directed evolution, neutral drift, sulfatase, superfamily
• Biological source: PSEUDOMONAS AERUGINOSA
• Total number of polymer chains: 2
• Total formula weight: 120657.29

Authors

Miton, C.M.,Jonas, S.,Mohammed, M.F.,Fischer, G.,Loo, B.v.,Kintses, B.,Hyvonen, M.,Tokuriki, N.,Hollfelder, F. (deposition date: 2014-04-08, release date: 2015-04-22, Last modification date: 2023-12-20)

• Primary citation: Miton, C.M.,Jonas, S.,Fischer, G.,Duarte, F.,Mohamed, M.F.,van Loo, B.,Kintses, B.,Kamerlin, S.C.L.,Tokuriki, N.,Hyvonen, M.,Hollfelder, F.; Evolutionary repurposing of a sulfatase: A new Michaelis complex leads to efficient transition state charge offset.; Proc. Natl. Acad. Sci. U.S.A., 115:E7293-E7302, 2018; PubMed: 30012610; DOI: 10.1073/pnas.1607817115

Experimental method

X-RAY DIFFRACTION (2.03 Å)