3ZXL

Engineering the active site of a GH43 glycoside hydrolase generates a biotechnologically significant enzyme that displays both endo- xylanase and exo-arabinofuranosidase activity

Summary for 3ZXL

• Entry DOI: 10.2210/pdb3zxl/pdb
• Related: 3ZXJ 3ZXK
• Descriptor: HIAXHD3, 2-[3-(2-HYDROXY-1,1-DIHYDROXYMETHYL-ETHYLAMINO)-PROPYLAMINO]-2-HYDROXYMETHYL-PROPANE-1,3-DIOL (3 entities in total)
• Functional Keywords: hydrolase, arabinosidase, xylosidase
• Biological source: HUMICOLA INSOLENS
• Total number of polymer chains: 2
• Total formula weight: 121427.10

Authors

McKee, L.S.,Pena, M.J.,Rogowski, A.,Jackson, A.,Lewis, R.J.,York, W.S.,Krogh, K.B.R.M.,Vikso-Nielsen, A.,Skjot, M.,Gilbert, H.J.,Marles-Wright, J. (deposition date: 2011-08-11, release date: 2012-04-18, Last modification date: 2023-12-20)

• Primary citation: Mckee, L.S.,Pena, M.J.,Rogowski, A.,Jackson, A.,Lewis, R.J.,York, W.S.,Krogh, K.B.R.M.,Vikso-Nielsen, A.,Skjot, M.,Gilbert, H.J.,Marles-Wright, J.; Introducing Endo-Xylanase Activity Into an Exo-Acting Arabinofuranosidase that Targets Side Chains.; Proc.Natl.Acad.Sci.USA, 109:6537-, 2012; PubMed: 22492980; DOI: 10.1073/PNAS.1117686109

Experimental method

X-RAY DIFFRACTION (1.871 Å)