3VDB

E. coli (lacZ) beta-galactosidase (N460T) in complex with galactonolactone

Summary for 3VDB

Entry DOI	10.2210/pdb3vdb/pdb
Related	1DP0 1JZ5 3VD3 3VD4 3VD5 3VD7 3VD9 3VDA 3VDC
Descriptor	Beta-galactosidase, D-galactonolactone, MAGNESIUM ION, ... (6 entities in total)
Functional Keywords	transition state stabilization, substrate binding, 2-stage binding, tim barrel (alpha/beta barrel), jelly-roll barrel, immunoglobulin, beta supersandwich, glycosidase, hydrolase
Biological source	Escherichia coli
Total number of polymer chains	4
Total formula weight	486192.44
Authors	Wheatley, R.W.,Kappelhoff, J.C.,Hahn, J.N.,Dugdale, M.L.,Dutkoski, M.J.,Tamman, S.D.,Fraser, M.E.,Huber, R.E. (deposition date: 2012-01-04, release date: 2012-04-11, Last modification date: 2023-09-13)
Primary citation	Wheatley, R.W.,Kappelhoff, J.C.,Hahn, J.N.,Dugdale, M.L.,Dutkoski, M.J.,Tamman, S.D.,Fraser, M.E.,Huber, R.E. Substitution for Asn460 cripples {beta}-galactosidase (Escherichia coli) by increasing substrate affinity and decreasing transition state stability. Arch.Biochem.Biophys., 521:51-61, 2012 Cited by PubMed: 22446164 DOI: 10.1016/j.abb.2012.03.014 PDB entries with the same primary citation
Experimental method	X-RAY DIFFRACTION (2.05 Å)

Structure validation