3VD5

E. coli (lacZ) beta-galactosidase (N460S)

Summary for 3VD5

• Entry DOI: 10.2210/pdb3vd5/pdb
• Related: 1DP0 3VD3 3VD4 3VD7 3VD9 3VDA 3VDB 3VDC
• Descriptor: Beta-galactosidase, MAGNESIUM ION, SODIUM ION, ... (5 entities in total)
• Functional Keywords: transition state stabilization, substrate binding, 2-stage binding, tim barrel (alpha/beta barrel), jelly-roll barrel, immunoglobulin, beta supersandwich, glycosidase, hydrolase
• Biological source: Escherichia coli
• Total number of polymer chains: 4
• Total formula weight: 481189.43

Authors

Wheatley, R.W.,Kappelhoff, J.C.,Hahn, J.N.,Dugdale, M.L.,Dutkoski, M.J.,Tamman, S.D.,Fraser, M.E.,Huber, R.E. (deposition date: 2012-01-04, release date: 2012-04-11, Last modification date: 2023-09-13)

• Primary citation: Wheatley, R.W.,Kappelhoff, J.C.,Hahn, J.N.,Dugdale, M.L.,Dutkoski, M.J.,Tamman, S.D.,Fraser, M.E.,Huber, R.E.; Substitution for Asn460 cripples {beta}-galactosidase (Escherichia coli) by increasing substrate affinity and decreasing transition state stability.; Arch.Biochem.Biophys., 521:51-61, 2012; PubMed: 22446164; DOI: 10.1016/j.abb.2012.03.014

Experimental method

X-RAY DIFFRACTION (2.7 Å)