3RWK

First crystal structure of an endo-inulinase, from Aspergillus ficuum: structural analysis and comparison with other GH32 enzymes.

Summary for 3RWK

Entry DOI	10.2210/pdb3rwk/pdb
Descriptor	Inulinase, alpha-D-mannopyranose-(1-3)-alpha-D-mannopyranose-(1-6)-alpha-D-mannopyranose, beta-D-mannopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose, ... (8 entities in total)
Functional Keywords	endo-inulinase, glycoside hydrolase family 32, catalytic mechanism, glycosidase hydrolase family 32, glycosylation, cytosol, hydrolase
Biological source	Aspergillus ficuum
Cellular location	Secreted: O94220
Total number of polymer chains	1
Total formula weight	57460.96
Authors	Michaux, C.,Pouyez, J.,Roussel, G.,Mayard, A.,Vandamme, A.M.,Housen, I.,Wouters, J. (deposition date: 2011-05-09, release date: 2012-07-18, Last modification date: 2020-07-29)
Primary citation	Pouyez, J.,Mayard, A.,Vandamme, A.M.,Roussel, G.,Perpete, E.A.,Wouters, J.,Housen, I.,Michaux, C. First crystal structure of an endo-inulinase, INU2, from Aspergillus ficuum: Discovery of an extra-pocket in the catalytic domain responsible for its endo-activity. Biochimie, 94:2423-2430, 2012 Cited by PubMed: 22750808 DOI: 10.1016/j.biochi.2012.06.020 PDB entries with the same primary citation
Experimental method	X-RAY DIFFRACTION (2.1 Å)

Structure validation