3ONW

Structure of a G-alpha-i1 mutant with enhanced affinity for the RGS14 GoLoco motif.

Summary for 3ONW

Entry DOI	10.2210/pdb3onw/pdb
Related	1kjy 2om2
Descriptor	Guanine nucleotide-binding protein G(i) subunit alpha-1, Regulator of G-protein signaling 14, GUANOSINE-5'-DIPHOSPHATE, ... (5 entities in total)
Functional Keywords	rgs14 goloco, rosetta, protein design, affinity enhancement, ras-like domain, all-helical domain, goloco motif, arginine finger, signaling protein, lipoprotein, transducer, guanine nucleotide dissociation inhibitor, gtp binding, nucleotide binding, adp-ribosylation
Biological source	Homo sapiens (human) More
Cellular location	Nucleus (By similarity): P63096 O43566
Total number of polymer chains	4
Total formula weight	84195.11
Authors	Bosch, D.,Kimple, A.J.,Sammond, D.W.,Miley, M.J.,Machius, M.,Kuhlman, B.,Willard, F.S.,Siderovski, D.P. (deposition date: 2010-08-30, release date: 2010-11-24, Last modification date: 2023-09-06)
Primary citation	Bosch, D.E.,Kimple, A.J.,Sammond, D.W.,Muller, R.E.,Miley, M.J.,Machius, M.,Kuhlman, B.,Willard, F.S.,Siderovski, D.P. Structural Determinants of Affinity Enhancement between GoLoco Motifs and G-Protein {alpha} Subunit Mutants. J.Biol.Chem., 286:3351-3358, 2011 Cited by PubMed: 21115486 DOI: 10.1074/jbc.M110.190496 PDB entries with the same primary citation
Experimental method	X-RAY DIFFRACTION (2.38 Å)

Structure validation