3N97

RNA polymerase alpha C-terminal domain (E. coli) and sigma region 4 (T. aq. mutant) bound to (UP,-35 element) DNA

Summary for 3N97

• Entry DOI: 10.2210/pdb3n97/pdb
• Related: 3K4G 3N4M
• Descriptor: RNA polymerase sigma factor, DNA-directed RNA polymerase subunit alpha, DNA (5'-D(*TP*GP*GP*AP*AP*AP*AP*AP*AP*GP*TP*AP*CP*TP*TP*GP*AP*CP*AP*TP*GP*G)-3'), ... (6 entities in total)
• Functional Keywords: protein-protein interactions, protein-dna interactions, transcription initiation, gene regulation-dna complex, gene regulation/dna
• Biological source: Thermus aquaticus; More
• Total number of polymer chains: 6
• Total formula weight: 49031.93

Authors

Lara-Gonzalez, S.,Birktoft, J.J.,Lawson, C.L. (deposition date: 2010-05-28, release date: 2011-06-08, Last modification date: 2023-09-06)

• Primary citation: Lara-Gonzalez, S.,Dantas Machado, A.C.,Rao, S.,Napoli, A.A.,Birktoft, J.,Di Felice, R.,Rohs, R.,Lawson, C.L.; The RNA Polymerase alpha Subunit Recognizes the DNA Shape of the Upstream Promoter Element.; Biochemistry, 59:4523-4532, 2020; PubMed: 33205945; DOI: 10.1021/acs.biochem.0c00571

Experimental method

X-RAY DIFFRACTION (3.252 Å)