3HJF

Crystal structure of T. thermophilus Argonaute E546 mutant protein complexed with DNA guide strand and 15-nt RNA target strand

Summary for 3HJF

• Entry DOI: 10.2210/pdb3hjf/pdb
• Related: 3DLB 3DLH 3HGE 3f73
• Descriptor: Argonaute, 5'-D(P*TP*GP*AP*GP*GP*TP*AP*GP*TP*AP*GP*GP*TP*TP*GP*TP*AP*TP*AP*GP*T)-3', 5'-R(*CP*AP*AP*CP*CP*UP*AP*CP*UP*AP*CP*CP*UP*CP*G)-3', ... (5 entities in total)
• Functional Keywords: argonaute, protein-dna_rna complex, nucleic acid binding protein-dna-rna complex, nucleic acid binding protein/dna/rna
• Biological source: Thermus thermophilus
• Total number of polymer chains: 3
• Total formula weight: 88027.18

Authors

Wang, Y.,Li, H.,Sheng, G.,Patel, D.J. (deposition date: 2009-05-21, release date: 2009-10-06, Last modification date: 2023-09-06)

• Primary citation: Wang, Y.,Juranek, S.,Li, H.,Sheng, G.,Wardle, G.S.,Tuschl, T.,Patel, D.J.; Nucleation, propagation and cleavage of target RNAs in Ago silencing complexes.; Nature, 461:754-761, 2009; PubMed: 19812667; DOI: 10.1038/nature08434

Experimental method

X-RAY DIFFRACTION (3.056 Å)