3FTN

Q165E/S254K Double Mutant Chimera of alcohol dehydrogenase by exchange of the cofactor binding domain res 153-295 of T. brockii ADH by C. beijerinckii ADH

Summary for 3FTN

Entry DOI	10.2210/pdb3ftn/pdb
Descriptor	NADP-dependent alcohol dehydrogenase, ZINC ION, ACETATE ION, ... (6 entities in total)
Functional Keywords	oxydoreductase, bacterial alcohol dehydrogenase, domain exchange, chimera, metal-binding, nadp, oxidoreductase, zinc
Biological source	Thermoanaerobacter brockii More
Total number of polymer chains	4
Total formula weight	152810.06
Authors	Frolow, F.,Goihberg, E.,Shimon, L.,Burstein, Y. (deposition date: 2009-01-13, release date: 2010-01-26, Last modification date: 2024-02-21)
Primary citation	Goihberg, E.,Peretz, M.,Tel-Or, S.,Dym, O.,Shimon, L.,Frolow, F.,Burstein, Y. Biochemical and structural properties of chimeras constructed by exchange of cofactor-binding domains in alcohol dehydrogenases from thermophilic and mesophilic microorganisms. Biochemistry, 49:1943-1953, 2010 Cited by PubMed: 20102159 DOI: 10.1021/bi901730x PDB entries with the same primary citation
Experimental method	X-RAY DIFFRACTION (2.192 Å)

Structure validation