2M5X

Novel method of protein purification for structural research. Example of ultra high resolution structure of SPI-2 inhibitor by X-ray and NMR spectroscopy.

Summary for 2M5X

• Entry DOI: 10.2210/pdb2m5x/pdb
• Related: 4hgu
• NMR Information: BMRB: 19085
• Descriptor: Silk protease inhibitor 2 (1 entity in total)
• Functional Keywords: serine protease inhibitor, kazal family, spi-2, hydrolase inhibitor
• Biological source: Galleria mellonella (Greater wax moth)
• Total number of polymer chains: 1
• Total formula weight: 4317.74

Authors

Lenarcic Zivkovic, M.,Dvornyk, A.,Kludkiewicz, B.,Kopera, E.,Zagorski-Ostoja, W.,Grzelak, K.,Zhukov, I.,Bal, W. (deposition date: 2013-03-12, release date: 2014-03-12, Last modification date: 2023-06-14)

• Primary citation: Kopera, E.,Bal, W.,Lenarcic Zivkovic, M.,Dvornyk, A.,Kludkiewicz, B.,Grzelak, K.,Zhukov, I.,Zagorski-Ostoja, W.,Jaskolski, M.,Krzywda, S.; Atomic resolution structure of a protein prepared by non-enzymatic His-tag removal. Crystallographic and NMR study of GmSPI-2 inhibitor.; Plos One, 9:e106936-e106936, 2014; PubMed: 25233114; DOI: 10.1371/journal.pone.0106936

Experimental method

SOLUTION NMR