2B0E

EcoRV Restriction Endonuclease/GAAUTC/Ca2+

Summary for 2B0E

• Entry DOI: 10.2210/pdb2b0e/pdb
• Related: 1AZ0 1BSU 2B0E
• Descriptor: 5'-D(*AP*AP*AP*GP*AP*AP*(DU)P*TP*CP*TP*T)-3', Type II restriction enzyme EcoRV, CALCIUM ION, ... (4 entities in total)
• Functional Keywords: protein-nucleic acid recognition, indirect readout, restriction enzyme, substrate specificity, noncognate, hydrolase-dna complex, hydrolase/dna
• Biological source: Escherichia coli
• Total number of polymer chains: 4
• Total formula weight: 64145.28

Authors

Hiller, D.A.,Rodriguez, A.M.,Perona, J.J. (deposition date: 2005-09-13, release date: 2005-09-27, Last modification date: 2024-02-14)

• Primary citation: Hiller, D.A.,Rodriguez, A.M.,Perona, J.J.; Non-cognate Enzyme-DNA Complex: Structural and Kinetic Analysis of EcoRV Endonuclease Bound to the EcoRI Recognition Site GAATTC; J.Mol.Biol., 354:121-136, 2005; PubMed: 16236314; DOI: 10.1016/j.jmb.2005.09.046

Experimental method

X-RAY DIFFRACTION (1.9 Å)