1MEP

Crystal Structure of Streptavidin Double Mutant S45A/D128A with Biotin: Cooperative Hydrogen-Bond Interactions in the Streptavidin-Biotin System.

Summary for 1MEP

Entry DOI	10.2210/pdb1mep/pdb
Related	1MK5 1SWE
Descriptor	Streptavidin, BIOTIN (3 entities in total)
Functional Keywords	homotetramer, biotin-binding protein
Biological source	Streptomyces avidinii
Cellular location	Secreted: P22629
Total number of polymer chains	4
Total formula weight	53862.55
Authors	Hyre, D.E.,Le Trong, I.,Merritt, E.A.,Stenkamp, R.E.,Green, N.M.,Stayton, P.S. (deposition date: 2002-08-08, release date: 2003-09-02, Last modification date: 2024-02-14)
Primary citation	Hyre, D.E.,Le Trong, I.,Merritt, E.A.,Eccleston, J.F.,Green, N.M.,Stenkamp, R.E.,Stayton, P.S. Cooperative hydrogen bond interactions in the streptavidin-biotin system Protein Sci., 15:459-467, 2006 Cited by PubMed: 16452627 DOI: 10.1110/ps.051970306 PDB entries with the same primary citation
Experimental method	X-RAY DIFFRACTION (1.65 Å)

Structure validation