1G2T

SOLUTION STRUCTURE OF EOTAXIN-3

> Summary

Summary for 1G2T

Related1G2S
DescriptorEOTAXIN-3
Functional Keywordsbeta-beta-beta-alpha helix, cytokine
Biological sourceHomo sapiens (human)
Total number of polymer chains1
Total molecular weight8412.93
Authors
Ye, J.,Mayer, K.L.,Mayer, M.R.,Stone, M.J. (deposition date: 2000-10-20, release date: 2002-03-13, modification date: 2009-02-24)
Primary citation
Ye, J.,Mayer, K.L.,Mayer, M.R.,Stone, M.J.
NMR solution structure and backbone dynamics of the CC chemokine eotaxin-3.
Biochemistry, 40:7820-7831, 2001
PubMed: 11425309
DOI: 10.1021/bi010252s
MImport into Mendeley
Experimental method
SOLUTION NMR
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Structure validation

ClashscoreRamachandran outliersSidechain outliers271.2%24.9%MetricValuePercentile RanksWorseBetterPercentile relative to all structuresPercentile relative to all NMR structures

More Asymmetric unit images

Molmil generated image of 1g2t
no rotation
Molmil generated image of 1g2t
rotated about x axis by 90°
Molmil generated image of 1g2t
rotated about y axis by 90°

> Structural details

Entity

Chain IDDescriptionTypeChain lengthFormula weightNumber of moleculesDB Name (Accession)Biological sourceDescriptive keywords
AEOTAXIN-3polymer718412.91
UniProt (Q9Y258)
Pfam (PF00048)
Homo sapiens (human)@PDBjSMALL INDUCIBLE CYTOKINE A26, MACROPHAGE INFLAMMATORY PROTEIN 4-ALPHA

Sequence viewer

Contents of the asymmetric unit

PolymersNumber of chains1
Total molecular weight8412.9
Non-Polymers*Number of molecules0
Total molecular weight0.0
All*Total molecular weight8412.9
*Water molecules are not included.

> Experimental details

Spectrometer

Experimental method:SOLUTION NMR

Spectrometer IDSpectrometer makerSpectrometer modelSpectrometer typeSpectrometer field strength
1VarianUNITY500

Experiment

experiment idconditions idsolution idExperiment type
1113D_15N-separated_NOESY
211HNHA
31213C, 15N simultaneously edited NOESY
4133D_13C-separated_NOESY
51413C-edited HSQC

NMR Sample

conditions idNMR sample pHNMR sample pressureNMR sample temperature
15ambient303

Conformers

Conformers Calculated Total Number100
Conformers Submitted Total Number20

> Functional details

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Functional Information from GO Data

ChainGOidnamespacecontents
A0005615cellular_componentextracellular space
A0048020molecular_functionCCR chemokine receptor binding
A0008009molecular_functionchemokine activity
A0007267biological_processcell-cell signaling
A0071346biological_processcellular response to interferon-gamma
A0071347biological_processcellular response to interleukin-1
A0071356biological_processcellular response to tumor necrosis factor
A0070098biological_processchemokine-mediated signaling pathway
A0006935biological_processchemotaxis
A0007186biological_processG-protein coupled receptor signaling pathway
A0006955biological_processimmune response
A0006954biological_processinflammatory response
A0048247biological_processlymphocyte chemotaxis
A0002548biological_processmonocyte chemotaxis
A0030593biological_processneutrophil chemotaxis
A0030838biological_processpositive regulation of actin filament polymerization
A0030335biological_processpositive regulation of cell migration
A0001938biological_processpositive regulation of endothelial cell proliferation
A0070374biological_processpositive regulation of ERK1 and ERK2 cascade
A0043547biological_processpositive regulation of GTPase activity
A0050729biological_processpositive regulation of inflammatory response
A0007165biological_processsignal transduction
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Functional Information from PDB Data

site_idNumber of ResiduesDetails
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Functional Information from PDB atom coordinates for the "HETATM" binding sites

site_idNumber of ResiduesDetails
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Functional Information from PROSITE/UniProt

site_idNumber of ResiduesDetails
PS004721Small cytokines (intercrine/chemokine) C-C subfamily signature. C-C-[LIFYTRQ]-x(5,8)-[LIR]-x(4)-[LIVMFA]-x(2)-[FYWECI]-x(5,8)-C-x(3,4)
ChainResidueDetails
ANA*

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Functional Information from SwissProt/UniProt

site_idNumber of ResiduesDetails
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Catalytic Information from CSA

site_idNumber of ResiduesDetails
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Catalytic Information from CATRES

site_idNumber of ResiduesDetails

> Sequence Neighbor

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