1EO4

ECORV BOUND TO MN2+ AND COGNATE DNA CONTAINING A 3'S SUBSTITION AT THE CLEAVAGE SITE

Summary for 1EO4

• Entry DOI: 10.2210/pdb1eo4/pdb
• Descriptor: DNA (5'-D(*CP*AP*AP*GP*AP*(TSP)P*AP*TP*CP*TP*T)-3'), TYPE II RESTRICTION ENZYME ECORV, MANGANESE (II) ION, ... (5 entities in total)
• Functional Keywords: protein-dna complex, restriction endonuclease, dna analog, hydrolase-dna complex, hydrolase/dna
• Biological source: Escherichia coli
• Total number of polymer chains: 4
• Total formula weight: 64472.05

Authors

Horton, N.C.,Connolly, B.A.,Perona, J.J. (deposition date: 2000-03-21, release date: 2000-04-04, Last modification date: 2024-02-07)

• Primary citation: Horton, N.C.,Connolly, B.A.,Perona, J.J.; Inhibition of EcoRV Endonuclease by Deoxyribo-3'-S-phosphorothiolates: A High-Resolution X-ray Crystallographic Study; J.Am.Chem.Soc., 122:3314-3324, 2000; DOI: 10.1021/ja993719j

Experimental method

X-RAY DIFFRACTION (1.9 Å)