1DLS

METHOTREXATE-RESISTANT VARIANTS OF HUMAN DIHYDROFOLATE REDUCTASE WITH SUBSTITUTION OF LEUCINE 22: KINETICS, CRYSTALLOGRAPHY AND POTENTIAL AS SELECTABLE MARKERS

1DLS の概要

• エントリーDOI: 10.2210/pdb1dls/pdb
• 分子名称: DIHYDROFOLATE REDUCTASE, NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE, METHOTREXATE, ... (4 entities in total)
• 機能のキーワード: oxido-reductase, oxidoreductase
• 由来する生物種: Homo sapiens (human)
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 22599.40

構造登録者

Cody, V.,Galitsky, N.,Luft, J.R.,Pangborn, W. (登録日: 1995-01-25, 公開日: 1995-04-20, 最終更新日: 2024-02-07)

• 主引用文献: Lewis, W.S.,Cody, V.,Galitsky, N.,Luft, J.R.,Pangborn, W.,Chunduru, S.K.,Spencer, H.T.,Appleman, J.R.,Blakley, R.L.; Methotrexate-resistant variants of human dihydrofolate reductase with substitutions of leucine 22. Kinetics, crystallography, and potential as selectable markers.; J.Biol.Chem., 270:5057-5064, 1995; PubMed: 7890613; DOI: 10.1074/jbc.270.10.5057

実験手法

X-RAY DIFFRACTION (2.3 Å)