6B00

Thermostabilized mutant of human carbonic anhydrase II - A65T L100H K154N L224S L240P A248T

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A	Carbonic anhydrase 2	polymer	266	30129.8	1	UniProt (P00918) Pfam (PF00194) In PDB	Homo sapiens (Human)	Carbonate dehydratase II,Carbonic anhydrase C,CAC,Carbonic anhydrase II,CA-II
2	A	GLYCEROL	non-polymer		92.1	1	Chemie (GOL)
3	A	ZINC ION	non-polymer		65.4	1	Chemie (ZN)
4		water	water		18.0	409	Chemie (HOH)

Sequence modifications

A: 3 - 261 (UniProt: P00918)

PDB	External Database	Details
Met 1	-	initiating methionine
Ala 2	-	expression tag
Thr 65	Ala 65	engineered mutation
His 100	Leu 100	engineered mutation
Asn 154	Lys 153	engineered mutation
Ser 224	Leu 223	engineered mutation
Pro 240	Leu 239	engineered mutation
Thr 248	Ala 247	engineered mutation
His 262	-	expression tag
His 263	-	expression tag
His 264	-	expression tag
His 265	-	expression tag
His 266	-	expression tag
His 267	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	1
	Total formula weight	30129.8
Non-Polymers*	Number of molecules	2
	Total formula weight	157.5
All*	Total formula weight	30287.3

*Water molecules are not included.