8G0Z
 
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8DTP
 | | Close state of T4 bacteriophage gp41 hexamer bound with single strand DNA | | Descriptor: | DNA (5'-D(P*TP*TP*TP*TP*TP*TP*TP*TP*TP*TP*TP*TP*TP*TP*TP*TP*TP*T)-3'), DnaB-like replicative helicase, MAGNESIUM ION, ... | | Authors: | Feng, X, Li, H. | | Deposit date: | 2022-07-26 | | Release date: | 2023-06-14 | | Last modified: | 2023-08-23 | | Method: | ELECTRON MICROSCOPY (2.7 Å) | | Cite: | Structural basis of the T4 bacteriophage primosome assembly and primer synthesis.
Nat Commun, 14, 2023
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8DUE
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8DVF
 | | T4 Bacteriophage primosome with single strand DNA, state 1 | | Descriptor: | DNA (5'-D(P*GP*GP*CP*TP*G)-3'), DNA (5'-D(P*TP*TP*TP*TP*TP*TP*TP*TP*TP*TP*TP*T)-3'), DNA primase, ... | | Authors: | Feng, X, Li, H. | | Deposit date: | 2022-07-28 | | Release date: | 2023-06-14 | | Last modified: | 2023-08-23 | | Method: | ELECTRON MICROSCOPY (3.3 Å) | | Cite: | Structural basis of the T4 bacteriophage primosome assembly and primer synthesis.
Nat Commun, 14, 2023
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8DW6
 | | T4 bacteriophage primosome with single-strand DNA, State 3 | | Descriptor: | DNA (5'-D(P*TP*TP*TP*TP*TP*TP*TP*TP*TP*TP*TP*T)-3'), DNA (70-MER), DNA primase, ... | | Authors: | Feng, X, Li, H. | | Deposit date: | 2022-07-31 | | Release date: | 2023-06-14 | | Last modified: | 2023-08-23 | | Method: | ELECTRON MICROSCOPY (3.5 Å) | | Cite: | Structural basis of the T4 bacteriophage primosome assembly and primer synthesis.
Nat Commun, 14, 2023
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8DUO
 | | DNA-free T4 Bacteriophage gp41 hexamer | | Descriptor: | DnaB-like replicative helicase, MAGNESIUM ION, PHOSPHOTHIOPHOSPHORIC ACID-ADENYLATE ESTER | | Authors: | Feng, X, Li, H. | | Deposit date: | 2022-07-27 | | Release date: | 2023-06-14 | | Last modified: | 2023-08-23 | | Method: | ELECTRON MICROSCOPY (5.7 Å) | | Cite: | Structural basis of the T4 bacteriophage primosome assembly and primer synthesis.
Nat Commun, 14, 2023
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8DVI
 | | T4 bacteriophage primosome with single strand DNA, State 2 | | Descriptor: | DNA (5'-D(P*TP*TP*TP*TP*TP*TP*TP*TP*TP*TP*TP*T)-3'), DNA (70-MER), DNA primase, ... | | Authors: | Feng, X, Li, H. | | Deposit date: | 2022-07-29 | | Release date: | 2023-06-14 | | Last modified: | 2023-08-23 | | Method: | ELECTRON MICROSCOPY (3.2 Å) | | Cite: | Structural basis of the T4 bacteriophage primosome assembly and primer synthesis.
Nat Commun, 14, 2023
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8GAO
 | | bacteriophage T4 stalled primosome with mutant gp41-E227Q | | Descriptor: | DNA (70-mer), DNA primase, DnaB-like replicative helicase, ... | | Authors: | Feng, X, Li, H. | | Deposit date: | 2023-02-23 | | Release date: | 2023-06-07 | | Last modified: | 2023-08-23 | | Method: | ELECTRON MICROSCOPY (4.1 Å) | | Cite: | Structural basis of the T4 bacteriophage primosome assembly and primer synthesis.
Nat Commun, 14, 2023
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8A3V
 | | Crystal structure of the Vibrio cholerae replicative helicase (VcDnaB) in complex with its loader protein (VcDciA) | | Descriptor: | ADENOSINE-5'-DIPHOSPHATE, DUF721 domain-containing protein, MAGNESIUM ION, ... | | Authors: | Walbott, H, Quevillon-Cheruel, S, Cargemel, C. | | Deposit date: | 2022-06-09 | | Release date: | 2023-02-15 | | Last modified: | 2024-02-07 | | Method: | X-RAY DIFFRACTION (2.9 Å) | | Cite: | The LH-DH module of bacterial replicative helicases is the common binding site for DciA and other helicase loaders.
Acta Crystallogr D Struct Biol, 79, 2023
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7T21
 | | E. coli DnaB bound to ssDNA and ADP-AlF4 | | Descriptor: | ADENOSINE-5'-DIPHOSPHATE, DNA (5'-D(*TP*TP*TP*TP*TP*TP*TP*TP*TP*TP*TP*TP*TP*TP*TP*TP*TP*TP*TP*T)-3'), MAGNESIUM ION, ... | | Authors: | Oakley, A.J, Xu, Z.Q. | | Deposit date: | 2021-12-02 | | Release date: | 2023-01-18 | | Method: | ELECTRON MICROSCOPY (5.4 Å) | | Cite: | Sequence of conformation changes of DnaB helicase during DNA unwinding and priming in Escherichia coli
To Be Published
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7T22
 | | E. coli DnaB bound to three DnaG C-terminal domains, ssDNA, ADP and AlF4 | | Descriptor: | ADENOSINE-5'-DIPHOSPHATE, DNA (5'-D(*TP*TP*TP*TP*TP*TP*TP*TP*TP*TP*TP*TP*TP*TP*TP*TP*TP*TP*TP*T)-3'), DNA primase, ... | | Authors: | Oakley, A.J, Xu, Z.Q. | | Deposit date: | 2021-12-02 | | Release date: | 2023-01-18 | | Last modified: | 2023-05-31 | | Method: | ELECTRON MICROSCOPY (4.2 Å) | | Cite: | Sequence of conformation changes of DnaB helicase during DNA unwinding and priming in Escherichia coli
To Be Published
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7T23
 | | E. coli DnaB bound to two DnaG C-terminal domains, ssDNA, ADP and AlF4 | | Descriptor: | ADENOSINE-5'-DIPHOSPHATE, DNA (5'-D(*TP*TP*TP*TP*TP*TP*TP*TP*TP*TP*TP*TP*TP*TP*TP*TP*TP*TP*TP*T)-3'), DNA primase, ... | | Authors: | Oakley, A.J, Xu, Z.Q. | | Deposit date: | 2021-12-02 | | Release date: | 2023-01-18 | | Last modified: | 2023-05-31 | | Method: | ELECTRON MICROSCOPY (4.2 Å) | | Cite: | Sequence of conformation changes of DnaB helicase during DNA unwinding and priming in Escherichia coli
To Be Published
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7T20
 | | E. coli DnaB bound to ssDNA and AMPPNP | | Descriptor: | DNA (5'-D(*TP*TP*TP*TP*TP*TP*TP*TP*TP*TP*TP*TP*TP*TP*TP*TP*TP*TP*TP*T)-3'), MAGNESIUM ION, PHOSPHOAMINOPHOSPHONIC ACID-ADENYLATE ESTER, ... | | Authors: | Oakley, A.J, Xu, Z.Q. | | Deposit date: | 2021-12-02 | | Release date: | 2022-12-14 | | Method: | ELECTRON MICROSCOPY (4.7 Å) | | Cite: | Sequence of conformation changes of DnaB helicase during DNA unwinding and priming in Escherichia coli
To Be Published
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7QXM
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6T66
 | | Crystal structure of the Vibrio cholerae replicative helicase (DnaB) with GDP-AlF4 | | Descriptor: | GUANOSINE-5'-DIPHOSPHATE, MAGNESIUM ION, Replicative DNA helicase, ... | | Authors: | Legrand, P, Quevillon-Cheruel, S, Li de la Sierra-Gallay, I, Walbott, H. | | Deposit date: | 2019-10-17 | | Release date: | 2021-04-28 | | Last modified: | 2024-01-24 | | Method: | X-RAY DIFFRACTION (3.9 Å) | | Cite: | Study of the DnaB:DciA interplay reveals insights into the primary mode of loading of the bacterial replicative helicase.
Nucleic Acids Res., 49, 2021
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6KZA
 | | Crystal structure of the complex of the interaction domains of E. coli DnaB helicase and DnaC helicase loader | | Descriptor: | DNA replication protein DnaC, Replicative DNA helicase | | Authors: | Nagata, K, Okada, A, Ohtsuka, J, Ohkuri, T, Akama, Y, Sakiyama, Y, Miyazaki, E, Horita, S, Katayama, T, Ueda, T, Tanokura, M. | | Deposit date: | 2019-09-23 | | Release date: | 2019-11-20 | | Last modified: | 2024-03-27 | | Method: | X-RAY DIFFRACTION (3.1 Å) | | Cite: | Crystal structure of the complex of the interaction domains of Escherichia coli DnaB helicase and DnaC helicase loader: structural basis implying a distortion-accumulation mechanism for the DnaB ring opening caused by DnaC binding.
J.Biochem., 167, 2020
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6N7T
 | | Structure of bacteriophage T7 E343Q mutant gp4 helicase-primase in complex with ssDNA, dTTP, AC dinucleotide and CTP (form III) | | Descriptor: | DNA (25-MER), DNA primase/helicase, MAGNESIUM ION, ... | | Authors: | Gao, Y, Cui, Y, Zhou, Z, Yang, W. | | Deposit date: | 2018-11-28 | | Release date: | 2019-03-06 | | Last modified: | 2024-03-20 | | Method: | ELECTRON MICROSCOPY (3.9 Å) | | Cite: | Structures and operating principles of the replisome.
Science, 363, 2019
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6N7S
 | | Structure of bacteriophage T7 E343Q mutant gp4 helicase-primase in complex with ssDNA, dTTP, AC dinucleotide and CTP (form II) | | Descriptor: | DNA (25-MER), DNA primase/helicase, MAGNESIUM ION, ... | | Authors: | Gao, Y, Cui, Y, Zhou, Z, Yang, W. | | Deposit date: | 2018-11-28 | | Release date: | 2019-03-06 | | Last modified: | 2024-03-20 | | Method: | ELECTRON MICROSCOPY (4.6 Å) | | Cite: | Structures and operating principles of the replisome.
Science, 363, 2019
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6N7N
 | | Structure of bacteriophage T7 E343Q mutant gp4 helicase-primase in complex with ssDNA, dTTP, AC dinucleotide and CTP (form I) | | Descriptor: | DNA (5'-D(P*TP*TP*TP*TP*TP*TP*TP*TP*TP*TP*TP*TP*TP*TP*T)-3'), DNA primase/helicase, MAGNESIUM ION, ... | | Authors: | Gao, Y, Cui, Y, Zhou, Z, Yang, W. | | Deposit date: | 2018-11-27 | | Release date: | 2019-03-06 | | Last modified: | 2024-03-20 | | Method: | ELECTRON MICROSCOPY (3.5 Å) | | Cite: | Structures and operating principles of the replisome.
Science, 363, 2019
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6N9W
 | | Structure of bacteriophage T7 lagging-strand DNA polymerase (D5A/E7A) and gp4 (helicase/primase) bound to DNA including RNA/DNA hybrid, and an incoming dTTP (LagS2) | | Descriptor: | DNA primase/helicase, DNA-directed DNA polymerase, MAGNESIUM ION, ... | | Authors: | Gao, Y, Cui, Y, Zhou, Z, Yang, W. | | Deposit date: | 2018-12-04 | | Release date: | 2019-03-06 | | Last modified: | 2019-12-18 | | Method: | ELECTRON MICROSCOPY (4 Å) | | Cite: | Structures and operating principles of the replisome.
Science, 363, 2019
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6N7V
 | | Structure of bacteriophage T7 gp4 (helicase-primase, E343Q mutant) in complex with ssDNA, dTTP, AC dinucleotide, and CTP (from multiple lead complexes) | | Descriptor: | DNA (93-MER), DNA primase/helicase, MAGNESIUM ION, ... | | Authors: | Gao, Y, Fox, T, Val, N, Yang, W. | | Deposit date: | 2018-11-28 | | Release date: | 2019-03-06 | | Last modified: | 2024-03-20 | | Method: | ELECTRON MICROSCOPY (3.8 Å) | | Cite: | Structures and operating principles of the replisome.
Science, 363, 2019
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6QEM
 | | E. coli DnaBC complex bound to ssDNA | | Descriptor: | ADENOSINE-5'-DIPHOSPHATE, DNA replication protein DnaC, MAGNESIUM ION, ... | | Authors: | Arias-Palomo, E, Puri, N, O'Shea Murray, V.L, Yan, Q, Berger, J.M. | | Deposit date: | 2019-01-08 | | Release date: | 2019-03-06 | | Last modified: | 2019-12-18 | | Method: | ELECTRON MICROSCOPY (3.4 Å) | | Cite: | Physical Basis for the Loading of a Bacterial Replicative Helicase onto DNA.
Mol.Cell, 74, 2019
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6QEL
 | | E. coli DnaBC apo complex | | Descriptor: | ADENOSINE-5'-DIPHOSPHATE, DNA replication protein dnaC, MAGNESIUM ION, ... | | Authors: | Arias-Palomo, E, Puri, N, O'Shea Murray, V.L, Yan, Q, Berger, J.M. | | Deposit date: | 2019-01-08 | | Release date: | 2019-03-06 | | Last modified: | 2019-12-18 | | Method: | ELECTRON MICROSCOPY (3.9 Å) | | Cite: | Physical Basis for the Loading of a Bacterial Replicative Helicase onto DNA.
Mol.Cell, 74, 2019
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6BBM
 | | Mechanisms of Opening and Closing of the Bacterial Replicative Helicase: The DnaB Helicase and Lambda P Helicase Loader Complex | | Descriptor: | ADENOSINE-5'-DIPHOSPHATE, Replication protein P, Replicative DNA helicase | | Authors: | Chase, J, Catalano, A, Noble, A.J, Eng, E.T, Olinares, P.D.B, Molloy, K, Pakotiprapha, D, Samuels, M, Chain, B, des Georges, A, Jeruzalmi, D. | | Deposit date: | 2017-10-18 | | Release date: | 2019-03-06 | | Last modified: | 2024-03-13 | | Method: | ELECTRON MICROSCOPY (4.1 Å) | | Cite: | Mechanisms of opening and closing of the bacterial replicative helicase.
Elife, 7, 2018
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6N9V
 | | Structure of bacteriophage T7 lagging-strand DNA polymerase (D5A/E7A) and gp4 (helicase/primase) bound to DNA including RNA/DNA hybrid, and an incoming dTTP (LagS1) | | Descriptor: | DNA primase/helicase, DNA-directed DNA polymerase, MAGNESIUM ION, ... | | Authors: | Gao, Y, Fox, T, Val, N, Yang, W. | | Deposit date: | 2018-12-04 | | Release date: | 2019-03-06 | | Last modified: | 2019-12-18 | | Method: | ELECTRON MICROSCOPY (4 Å) | | Cite: | Structures and operating principles of the replisome.
Science, 363, 2019
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