5T2U

short chain dehydrogenase/reductase family protein

Experimental procedure

実験手法	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID23-2
Synchrotron site	ESRF
Beamline	ID23-2
Temperature [K]	100
Detector technology	CCD
Collection date	2016-02-07
Detector	MARMOSAIC 225 mm CCD
Wavelength(s)	0.873
Spacegroup name	P 21 21 21
格子定数 [Å]	65.010, 116.110, 132.870
格子定数 [度]	90.00, 90.00, 90.00

精密化法

残基	19.910 - 2.200
R因子	0.1651
Rwork	0.163
R-free	0.21770
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5t2v
結合長の平均二乗偏差(RMSD) [Å]	0.003
結合角の平均二乗偏差(RMSD) [度]	0.693
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX ((1.10.1_2155: ???))

Quality characteristics

	Overall	Outer shell
分解能 [Å] (低)	19.910	2.270
分解能 [Å] (高)	2.200	2.200
Rmeas	0.176
独立反射数	51201
<I/σ(I)>	8.2	2.28
完全性 [%]	98.7	99.9
冗長性	3.7	3.7

結晶化条件

結晶ID	方法	pH	温度	溶液条件
1	VAPOR DIFFUSION, SITTING DROP		291	0.2M Na citrate tribasic dehydrate, 20% peg3350