5JGN

Spin-Labeled T4 Lysozyme Construct I9V1

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU FR-E SUPERBRIGHT
Temperature [K]	100
Detector technology	CCD
Collection date	2015-09-14
Detector	RIGAKU
Wavelength(s)	1.54178
Spacegroup name	P 32 2 1
Unit cell lengths	59.820, 59.820, 95.672
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	29.910 - 1.534
R-factor	0.153
Rwork	0.152
R-free	0.17170
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1c6t
RMSD bond length	0.006
RMSD bond angle	0.801
Data reduction software	xia2
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX ((1.10.1_2155: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	29.910
High resolution limit [Å]	1.534	1.534
Number of reflections	30273
<I/σ(I)>	20.09
Completeness [%]	100.0
Redundancy	2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		297	sodium/potassium phosphate, sodium chloride, hexane-1,6-diol, 2-propanol