5HDF
Hydrolase SeMet-StnA
Experimental procedure
| 実験手法 | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL17U |
| Synchrotron site | SSRF |
| Beamline | BL17U |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2014-05-27 |
| Detector | RAYONIX MX-225 |
| Wavelength(s) | 0.9819 |
| Spacegroup name | C 1 2 1 |
| 格子定数 [Å] | 178.420, 81.970, 118.693 |
| 格子定数 [度] | 90.00, 126.75, 90.00 |
精密化法
| 残基 | 50.000 - 2.710 |
| R因子 | 0.19366 |
| Rwork | 0.192 |
| R-free | 0.21957 |
| Structure solution method | SAD |
| 結合長の平均二乗偏差(RMSD) [Å] | 0.017 |
| 結合角の平均二乗偏差(RMSD) [度] | 1.250 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHENIX |
| Refinement software | REFMAC (5.8.0103) |
Quality characteristics
| Overall | Outer shell | |
| 分解能 [Å] (低) | 50.000 | 2.800 |
| 分解能 [Å] (高) | 2.710 | 2.700 |
| Rmerge_l_obs | 0.111 | 0.300 |
| 独立反射数 | 37623 | |
| <I/σ(I)> | 14.55 | 6.08 |
| 完全性 [%] | 99.9 | 99.9 |
| 冗長性 | 3.8 | 3.8 |
結晶化条件
| 結晶ID | 方法 | pH | 温度 | 溶液条件 |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5 | 287 | 30% 2-propanol, 0.1 M Sodium citrate pH 5.0,12% PEG 3350 |
