4M6D
Crystal structure of the aptamer minF-lysozyme complex.
Experimental procedure
Experimental method | SAD |
Source type | SYNCHROTRON |
Source details | NSLS BEAMLINE X29A |
Synchrotron site | NSLS |
Beamline | X29A |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2013-01-18 |
Detector | ADSC QUANTUM 315 |
Wavelength(s) | 1.075 |
Spacegroup name | P 1 |
Unit cell lengths | 43.914, 132.289, 131.539 |
Unit cell angles | 118.59, 96.39, 96.27 |
Refinement procedure
Resolution | 38.040 - 2.680 |
R-factor | 0.16832 |
Rwork | 0.165 |
R-free | 0.21600 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4m4o |
RMSD bond length | 0.013 |
RMSD bond angle | 1.421 |
Data reduction software | HKL-2000 |
Data scaling software | SCALEPACK |
Phasing software | PHASER (2.5.1) |
Refinement software | REFMAC |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 50.000 | 50.000 | 2.750 |
High resolution limit [Å] | 2.680 | 7.320 | 2.700 |
Rmerge | 0.067 | 0.037 | 0.629 |
Number of reflections | 67209 | ||
<I/σ(I)> | 12.1 | ||
Completeness [%] | 94.9 | 90.6 | 90.9 |
Redundancy | 1.9 | 1.9 | 1.8 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 7 | 298 | 1.1M Malonic Acid, 0.15 M Ammonium Citrate Tribasic, 0.072 M Succinic Acid, 0.18 M DL-Malic Acid, 0.24 M Sodium Acetate, 0.3 M Sodium Formate, 0.096 M Ammonium Tartrate Dibasic, Final pH 7.0, VAPOR DIFFUSION, SITTING DROP, temperature 298K |