3LEX
2F5 Epitope scaffold elicited anti-HIV-1 monoclonal antibody 11F10 in complex with HIV-1 GP41
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 22-BM |
Synchrotron site | APS |
Beamline | 22-BM |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2009-04-02 |
Detector | MARMOSAIC 300 mm CCD |
Wavelength(s) | 1.000 |
Spacegroup name | P 1 |
Unit cell lengths | 34.543, 70.962, 78.265 |
Unit cell angles | 90.08, 90.02, 90.29 |
Refinement procedure
Resolution | 25.900 - 1.970 |
R-factor | 0.166 |
Rwork | 0.165 |
R-free | 0.19800 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3ley |
RMSD bond length | 0.009 |
RMSD bond angle | 0.892 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHASER |
Refinement software | PHENIX |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 25.900 | 2.040 |
High resolution limit [Å] | 1.960 | 1.970 |
Number of reflections | 49925 | |
<I/σ(I)> | 33 | 12.9 |
Completeness [%] | 94.6 | 94.7 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 293 | 16% PEG 4000, 0.08 M CH3COONA, 0.04 M, TRIS PH 8.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K |