1RZ8
CRYSTAL STRUCTURE OF HUMAN ANTI-HIV-1 GP120-REACTIVE ANTIBODY 17B
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 1996-01-18 |
| Detector | RIGAKU RAXIS |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 63.212, 117.460, 154.813 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 20.000 * - 2.300 |
| R-factor | 0.2121 |
| Rwork | 0.212 |
| R-free | 0.26200 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1bbj |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.408 * |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 2.280 |
| High resolution limit [Å] | 2.200 | 2.200 |
| Rmerge | 0.086 * | |
| Total number of observations | 228323 * | |
| Number of reflections | 55222 | |
| Completeness [%] | 93.1 * | 70.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 * | 293 | 50% saturated ammonium sulfate, 10% 2-methyl-2,4-pentanediol, 0.1M imidazole, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | Tris-HCl | 2.5 (mM) | |
| 2 | 1 | drop | 0.35 (M) | ||
| 3 | 1 | drop | 0.02 (%(v/v)) | pH7.0 | |
| 4 | 1 | drop | protein | 4-8 (mg/ml) | |
| 5 | 1 | reservoir | ammonium sulfate | 50 (%sat) | |
| 6 | 1 | reservoir | MPD | 10 (%) | |
| 7 | 1 | reservoir | imidazole | 0.1 (M) | pH6.5 |
