1JB6
Crystal Structure of Dimerization Domain (1-33) of HNF-1alpha
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2000-03-24 |
| Detector | SBC-2 |
| Wavelength(s) | 0.9794, 0.9800, 1.0030 |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 28.420, 42.190, 42.430 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 40.000 - 1.700 |
| R-factor | 0.231 * |
| Rwork | 0.231 |
| R-free | 0.24500 |
| Structure solution method | MAD |
| RMSD bond length | 0.004 |
| RMSD bond angle | 0.800 |
| Data reduction software | d*TREK ((D*TREK)) |
| Data scaling software | HKL-2000 |
| Phasing software | CNS |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 40.000 | 1.760 |
| High resolution limit [Å] | 1.700 | 1.700 |
| Number of reflections | 5245 | |
| Completeness [%] | 88.0 | |
| Redundancy | 6 | 5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 * | 4 * | MPD, Tris-HCl, DTT, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 277K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | peptide | 3 (mM) | |
| 2 | 1 | drop | Tris-HCl | 50 (mM) | |
| 3 | 1 | drop | dithiothreitol | 2 (mM) | |
| 4 | 1 | reservoir | MPD | 20 (%(v/v)) | |
| 5 | 1 | reservoir | Tris-HCl | 50 (mM) | |
| 6 | 1 | reservoir | dithiothreitol | 1 (mM) |
