HUMAN GROWTH HORMONE MUTANT WITH PHE 10 REPLACED BY ALA, MET 14 REPLACED BY TRP, HIS 18 REPLACED BY ASP, HIS 21 REPLACED BY ASN, LYS 41 REPLACED BY ILE, TYR 42 REPLACED BY HIS, LEU 45 REPLACED BY TRP, GLN 46 REPLACED BY TRP, PHE 54 REPLACED BY PRO, ARG 64 REPLACED BY LYS, ARG 167 REPLACED ASN, ASP 171 REPLACED BY SER, GLU 174 REPLACED BY SER, PHE 176 REPLACED BY TYR, ILE 179 REPLACED BY THR (F10A, M14W,H18D,H21N,K41I,Y42H,L45W,Q46W,F54P,R64K,R167N,D171S, E174S,F176Y,I179T)
Ultsch, M.H., Somers, W.S., Kossiakoff, A.A., De Vos, A.M.
The crystal structure of affinity-matured human growth hormone at 2 A resolution. J.Mol.Biol., 236, 1994
PHAGE DISPLAY DERIVED VARIANT HUMAN GROWTH HORMONE, EXTRACELLULAR DOMAIN HUMAN GROWTH HORMONE RECEPTOR (1-238)
Schiffer, C.A., Ultsch, M., Walsh, S., Somers, W., De Vos, A.M., Kossiakoff, A.A.
Structure of a Phage Display Derived Variant of Human Growth Hormone Complexed to Two Copies of the Extracellular Domain of its Receptor: Evidence for Strong Structural Coupling between Receptor Binding Sites J.Mol.Biol., 316, 2002