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- PDB-4uom: Electron Cryo-microscopy of Venezuelan Equine Encephalitis Virus ... -

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Entry
Database: PDB / ID: 4uom
TitleElectron Cryo-microscopy of Venezuelan Equine Encephalitis Virus TC- 83 in complex with neutralizing antibody Fab F5
Components
  • FAB FRAGMENT HEAVY CHAINFragment antigen-binding
  • FAB FRAGMENT LIGHT CHAINFragment antigen-binding
KeywordsVIRAL PROTEIN / ALPHAVIRUS / VENEZUELAN / ANTIBODY NEUTRALIZATION / FAB
Biological speciesHOMO SAPIENS (human)
MethodELECTRON MICROSCOPY / single particle reconstruction / cryo EM / Resolution: 17 Å
AuthorsPorta, J. / Jose, J. / Roehrig, J.T. / Blair, C.D. / Kuhn, R.J. / Rossmann, M.G.
CitationJournal: J Virol / Year: 2014
Title: Locking and blocking the viral landscape of an alphavirus with neutralizing antibodies.
Authors: Jason Porta / Joyce Jose / John T Roehrig / Carol D Blair / Richard J Kuhn / Michael G Rossmann /
Abstract: Alphaviruses are serious, sometimes lethal human pathogens that belong to the family Togaviridae. The structures of human Venezuelan equine encephalitis virus (VEEV), an alphavirus, in complex with ...Alphaviruses are serious, sometimes lethal human pathogens that belong to the family Togaviridae. The structures of human Venezuelan equine encephalitis virus (VEEV), an alphavirus, in complex with two strongly neutralizing antibody Fab fragments (F5 and 3B4C-4) have been determined using a combination of cryo-electron microscopy and homology modeling. We characterize these monoclonal antibody Fab fragments, which are known to abrogate VEEV infectivity by binding to the E2 (envelope) surface glycoprotein. Both of these antibody Fab fragments cross-link the surface E2 glycoproteins and therefore probably inhibit infectivity by blocking the conformational changes that are required for making the virus fusogenic. The F5 Fab fragment cross-links E2 proteins within one trimeric spike, whereas the 3B4C-4 Fab fragment cross-links E2 proteins from neighboring spikes. Furthermore, F5 probably blocks the receptor-binding site, whereas 3B4C-4 sterically hinders the exposure of the fusion loop at the end of the E2 B-domain.
IMPORTANCE: Alphaviral infections are transmitted mainly by mosquitoes. Venezuelan equine encephalitis virus (VEEV) is an alphavirus with a wide distribution across the globe. No effective vaccines ...IMPORTANCE: Alphaviral infections are transmitted mainly by mosquitoes. Venezuelan equine encephalitis virus (VEEV) is an alphavirus with a wide distribution across the globe. No effective vaccines exist for alphaviral infections. Therefore, a better understanding of VEEV and its associated neutralizing antibodies will help with the development of effective drugs and vaccines.
History
DepositionJun 5, 2014Deposition site: PDBE / Processing site: PDBE
Revision 1.0Oct 15, 2014Provider: repository / Type: Initial release
Revision 1.1Aug 23, 2017Group: Data collection / Category: em_software / Item: _em_software.image_processing_id

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  • Deposited structure unit
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  • Simplified surface model + fitted atomic model
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Structure viewerMolecule:
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Assembly

Deposited unit
H: FAB FRAGMENT HEAVY CHAIN
L: FAB FRAGMENT LIGHT CHAIN


Theoretical massNumber of molelcules
Total (without water)45,5532
Polymers45,5532
Non-polymers00
Water0
1


  • Idetical with deposited unit
  • defined by software
TypeNameSymmetry operationNumber
identity operation1_555x,y,z1
MethodPISA

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Components

#1: Antibody FAB FRAGMENT HEAVY CHAIN / Fragment antigen-binding


Mass: 23376.186 Da / Num. of mol.: 1
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) HOMO SAPIENS (human) / Production host: ESCHERICHIA COLI (E. coli) / Strain (production host): BL21
#2: Antibody FAB FRAGMENT LIGHT CHAIN / Fragment antigen-binding


Mass: 22177.174 Da / Num. of mol.: 1
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) HOMO SAPIENS (human) / Production host: ESCHERICHIA COLI (E. coli) / Strain (production host): BL21

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Experimental details

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Experiment

ExperimentMethod: ELECTRON MICROSCOPY
EM experimentAggregation state: PARTICLE / 3D reconstruction method: single particle reconstruction

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Sample preparation

ComponentName: VENEZUELAN EQUINE ENCEPHALITIS VIRUS STRAIN TC-83 COMPLEXED TO FAB F5
Type: VIRUS
Buffer solutionName: 0.5 M NACL, 20 MM TRIS PH 7.6, 5 MM EDTA / pH: 7.6 / Details: 0.5 M NACL, 20 MM TRIS PH 7.6, 5 MM EDTA
SpecimenConc.: 1.5 mg/ml / Embedding applied: NO / Shadowing applied: NO / Staining applied: NO / Vitrification applied: YES
Specimen supportDetails: HOLEY CARBON
VitrificationInstrument: HOMEMADE PLUNGER / Cryogen name: ETHANE / Details: LIQUID ETHANE

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Electron microscopy imaging

Experimental equipment
Model: Titan Krios / Image courtesy: FEI Company
MicroscopyModel: FEI TITAN KRIOS / Date: Jul 14, 2012
Electron gunElectron source: FIELD EMISSION GUN / Accelerating voltage: 300 kV / Illumination mode: SPOT SCAN
Electron lensMode: BRIGHT FIELDBright-field microscopy / Nominal magnification: 59000 X / Calibrated magnification: 60521 X / Nominal defocus max: 3000 nm / Nominal defocus min: 1500 nm / Cs: 2.7 mm
Specimen holderTemperature: 100 K
Image recordingElectron dose: 24 e/Å2 / Film or detector model: KODAK SO-163 FILM
Image scansNum. digital images: 112
Radiation wavelengthRelative weight: 1

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Processing

EM software
IDNameVersionCategory
1EMAN13D reconstruction
2EMAN23D reconstruction
CTF correctionDetails: EACH IMAGE
SymmetryPoint symmetry: I (icosahedral)
3D reconstructionMethod: COMMON LINES / Resolution: 17 Å / Num. of particles: 1389 / Nominal pixel size: 1.07 Å / Actual pixel size: 1.11 Å
Details: SUBMISSION BASED ON EXPERIMENTAL DATA FROM EMDB EMD-2645. (DEPOSITION ID: 12481).
Symmetry type: POINT
Atomic model buildingProtocol: OTHER / Space: REAL / Details: REFINEMENT PROTOCOL--HOMOLOGY
RefinementHighest resolution: 17 Å
Refinement stepCycle: LAST / Highest resolution: 17 Å
ProteinNucleic acidLigandSolventTotal
Num. atoms3206 0 0 0 3206

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