+Open data
-Basic information
Entry | Database: PDB / ID: 2rec | ||||||
---|---|---|---|---|---|---|---|
Title | RECA HEXAMER MODEL, ELECTRON MICROSCOPY | ||||||
Components | RECA | ||||||
Keywords | HELICASE / DNA DAMAGE / DNA RECOMBINATION / SOS RESPONSE / ATP-BINDING / DNA-BINDING | ||||||
Function / homology | Function and homology information DNA polymerase V complex / homologous recombination / recombinational repair / SOS response / ATP-dependent DNA damage sensor activity / response to ionizing radiation / translesion synthesis / ATP-dependent activity, acting on DNA / cell motility / single-stranded DNA binding ...DNA polymerase V complex / homologous recombination / recombinational repair / SOS response / ATP-dependent DNA damage sensor activity / response to ionizing radiation / translesion synthesis / ATP-dependent activity, acting on DNA / cell motility / single-stranded DNA binding / DNA-binding transcription factor binding / DNA recombination / damaged DNA binding / DNA damage response / ATP hydrolysis activity / ATP binding / cytoplasm Similarity search - Function | ||||||
Biological species | Escherichia coli (E. coli) | ||||||
Method | ELECTRON MICROSCOPY / helical reconstruction / negative staining | ||||||
Authors | Yu, X. / Egelman, E.H. | ||||||
Citation | Journal: Nat Struct Biol / Year: 1997 Title: The RecA hexamer is a structural homologue of ring helicases. Abstract: The RecA protein forms a hexameric ring that is similar to the core of the F1-ATPase. Several lines of evidence suggest that this hexamer may be a structural homologue of ring helicases. | ||||||
History |
|
-Structure visualization
Movie |
Movie viewer |
---|---|
Structure viewer | Molecule: MolmilJmol/JSmol |
-Downloads & links
-Download
PDBx/mmCIF format | 2rec.cif.gz | 55.6 KB | Display | PDBx/mmCIF format |
---|---|---|---|---|
PDB format | pdb2rec.ent.gz | 34.9 KB | Display | PDB format |
PDBx/mmJSON format | 2rec.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/re/2rec ftp://data.pdbj.org/pub/pdb/validation_reports/re/2rec | HTTPS FTP |
---|
-Related structure data
Similar structure data |
---|
-Links
-Assembly
Deposited unit |
|
---|---|
1 |
|
-Components
#1: Protein | Mass: 38016.277 Da / Num. of mol.: 6 / Source method: isolated from a natural source / Source: (natural) Escherichia coli (E. coli) / References: UniProt: P0A7G6 |
---|
-Experimental details
-Experiment
Experiment | Method: ELECTRON MICROSCOPY |
---|---|
EM experiment | Aggregation state: FILAMENT / 3D reconstruction method: helical reconstruction |
-Sample preparation
Component | Name: RecA filament / Type: COMPLEX |
---|---|
Specimen | Embedding applied: NO / Shadowing applied: NO / Staining applied: YES / Vitrification applied: NO |
EM staining | Type: NEGATIVE / Material: uranyl acetate |
Crystal grow | *PLUS Method: other |
-Electron microscopy imaging
Microscopy | Model: JEOL 1200EXII |
---|---|
Electron gun | Accelerating voltage: 80 kV / Illumination mode: FLOOD BEAM |
Electron lens | Mode: BRIGHT FIELDBright-field microscopy |
Detector | Date: Sep 1, 1996 |
-Processing
Software | Name: XTALVIEW / Classification: refinement | ||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Refinement step | Cycle: LAST
|