the National Science and Technology Major Projects
2012ZX10001-009
China
the National Science and Technology Major Projects
2016ZX10001-004
China
Citation
Journal: Sci Rep / Year: 2016 Title: Potent neutralizing monoclonal antibodies against Ebola virus infection. Authors: Qi Zhang / Miao Gui / Xuefeng Niu / Shihua He / Ruoke Wang / Yupeng Feng / Andrea Kroeker / Yanan Zuo / Hua Wang / Ying Wang / Jiade Li / Chufang Li / Yi Shi / Xuanling Shi / George F Gao / ...Authors: Qi Zhang / Miao Gui / Xuefeng Niu / Shihua He / Ruoke Wang / Yupeng Feng / Andrea Kroeker / Yanan Zuo / Hua Wang / Ying Wang / Jiade Li / Chufang Li / Yi Shi / Xuanling Shi / George F Gao / Ye Xiang / Xiangguo Qiu / Ling Chen / Linqi Zhang / Abstract: Ebola virus infections cause a deadly hemorrhagic disease for which no vaccines or therapeutics has received regulatory approval. Here we show isolation of three (Q206, Q314 and Q411) neutralizing ...Ebola virus infections cause a deadly hemorrhagic disease for which no vaccines or therapeutics has received regulatory approval. Here we show isolation of three (Q206, Q314 and Q411) neutralizing monoclonal antibodies (mAbs) against the surface glycoprotein (GP) of Ebola virus identified in West Africa in 2014 through sequential immunization of Chinese rhesus macaques and antigen-specific single B cell sorting. These mAbs demonstrated potent neutralizing activities against both pseudo and live Ebola virus independent of complement. Biochemical, single particle EM, and mutagenesis analysis suggested Q206 and Q411 recognized novel epitopes in the head while Q314 targeted the glycan cap in the GP1 subunit. Q206 and Q411 appeared to influence GP binding to its receptor NPC1. Treatment with these mAbs provided partial but significant protection against disease in a mouse model of Ebola virus infection. These novel mAbs could serve as promising candidates for prophylactic and therapeutic interventions against Ebola virus infection.
History
Deposition
Apr 21, 2016
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Header (metadata) release
Jun 1, 2016
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Map release
Jun 1, 2016
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Update
Nov 30, 2022
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Current status
Nov 30, 2022
Processing site: PDBj / Status: Released
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