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- EMDB-6593: CSF_trimer in a post-fusion or intermediate conformation bound to... -

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Basic information

Entry
Database: EMDB / ID: EMD-6593
TitleCSF_trimer in a post-fusion or intermediate conformation bound to the bnAb PGV04
Map dataCSF_trimer_post_fusion bound to bnAb PGV04
Sample
  • Sample: CSF trimer post-fusion state or intermediate state bound to PGV04
  • Protein or peptide: CSF
  • Protein or peptide: PGV04
KeywordsHIV / envelope / glycoprotein / trimer
Biological speciesHuman immunodeficiency virus / Homo sapiens (human)
Methodsingle particle reconstruction / negative staining / Resolution: 20.0 Å
AuthorsKong L / He L / de Val N / Morris CD / Vora N / Azadnia P / Sok D / Zhou B / Burton DR / Ward AB ...Kong L / He L / de Val N / Morris CD / Vora N / Azadnia P / Sok D / Zhou B / Burton DR / Ward AB / Wilson IA / Zhu J
CitationJournal: Nat Commun / Year: 2016
Title: Uncleaved prefusion-optimized gp140 trimers derived from analysis of HIV-1 envelope metastability.
Authors: Leopold Kong / Linling He / Natalia de Val / Nemil Vora / Charles D Morris / Parisa Azadnia / Devin Sok / Bin Zhou / Dennis R Burton / Andrew B Ward / Ian A Wilson / Jiang Zhu /
Abstract: The trimeric HIV-1 envelope glycoprotein (Env) is critical for host immune recognition and neutralization. Despite advances in trimer design, the roots of Env trimer metastability remain elusive. ...The trimeric HIV-1 envelope glycoprotein (Env) is critical for host immune recognition and neutralization. Despite advances in trimer design, the roots of Env trimer metastability remain elusive. Here we investigate the contribution of two Env regions to metastability. First, we computationally redesign a largely disordered bend in heptad region 1 (HR1) of SOSIP trimers that connects the long, central HR1 helix to the fusion peptide, substantially improving the yield of soluble, well-folded trimers. Structural and antigenic analyses of two distinct HR1 redesigns confirm that redesigned Env closely mimics the native, prefusion trimer with a more stable gp41. Next, we replace the cleavage site between gp120 and gp41 with various linkers in the context of an HR1 redesign. Electron microscopy reveals a potential fusion intermediate state for uncleaved trimers containing short but not long linkers. Together, these results outline a general approach for stabilization of Env trimers from diverse HIV-1 strains.
History
DepositionFeb 11, 2016-
Header (metadata) releaseApr 27, 2016-
Map releaseJul 13, 2016-
UpdateJul 20, 2016-
Current statusJul 20, 2016Processing site: RCSB / Status: Released

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Structure visualization

Movie
  • Surface view with section colored by density value
  • Surface level: 22.2
  • Imaged by UCSF Chimera
  • Download
  • Surface view colored by cylindrical radius
  • Surface level: 22.2
  • Imaged by UCSF Chimera
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Movie viewer
Structure viewerEM map:
SurfViewMolmilJmol/JSmol
Supplemental images

400_6593.gif

Downloads & links

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Map

FileDownload / File: emd_6593.map.gz / Format: CCP4 / Size: 1.9 MB / Type: IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES)
AnnotationCSF_trimer_post_fusion bound to bnAb PGV04
Voxel sizeX=Y=Z: 4.1 Å
Density
Contour LevelBy AUTHOR: 22.199999999999999 / Movie #1: 22.2
Minimum - Maximum-22.016847609999999 - 59.357971190000001
Average (Standard dev.)0.93234384 (±6.00735807)
SymmetrySpace group: 1
Details

EMDB XML:

Map geometry
Axis orderXYZ
Origin000
Dimensions808080
Spacing808080
CellA=B=C: 328.0 Å
α=β=γ: 90.0 °

CCP4 map header:

modeImage stored as Reals
Å/pix. X/Y/Z4.14.14.1
M x/y/z808080
origin x/y/z0.0000.0000.000
length x/y/z328.000328.000328.000
α/β/γ90.00090.00090.000
MAP C/R/S123
start NC/NR/NS000
NC/NR/NS808080
D min/max/mean-22.01759.3580.932

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Supplemental data

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Sample components

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Entire : CSF trimer post-fusion state or intermediate state bound to PGV04

EntireName: CSF trimer post-fusion state or intermediate state bound to PGV04
Components
  • Sample: CSF trimer post-fusion state or intermediate state bound to PGV04
  • Protein or peptide: CSF
  • Protein or peptide: PGV04

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Supramolecule #1000: CSF trimer post-fusion state or intermediate state bound to PGV04

SupramoleculeName: CSF trimer post-fusion state or intermediate state bound to PGV04
type: sample / ID: 1000 / Oligomeric state: Trimer bound to three Fabs / Number unique components: 2
Molecular weightTheoretical: 570 KDa

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Macromolecule #1: CSF

MacromoleculeName: CSF / type: protein_or_peptide / ID: 1
Details: Heterodimer of gp120 and gp41 assembles into a trimer.
Number of copies: 2 / Oligomeric state: trimer / Recombinant expression: Yes
Source (natural)Organism: Human immunodeficiency virus
Molecular weightExperimental: 570 KDa / Theoretical: 570 KDa
Recombinant expressionOrganism: Homo sapiens (human) / Recombinant strain: HEK 293F / Recombinant cell: 293F

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Macromolecule #2: PGV04

MacromoleculeName: PGV04 / type: protein_or_peptide / ID: 2 / Name.synonym: Fab / Recombinant expression: Yes
Source (natural)Organism: Homo sapiens (human) / synonym: Human
Recombinant expressionOrganism: Homo sapiens (human) / Recombinant cell: HEK 293F

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Experimental details

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Structure determination

Methodnegative staining
Processingsingle particle reconstruction
Aggregation stateparticle

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Sample preparation

Concentration0.02 mg/mL
BufferpH: 7.4 / Details: 50 mM Tris-HCl, 150 mM NaCl
StainingType: NEGATIVE
Details: Grids were glow-discharged at 20 mA for 30 seconds and stained with 2% uranyl formate for 40 seconds.
GridDetails: 400 Cu mesh grids
VitrificationCryogen name: NONE / Instrument: OTHER

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Electron microscopy

MicroscopeFEI TECNAI SPIRIT
Electron beamAcceleration voltage: 120 kV / Electron source: LAB6
Electron opticsCalibrated magnification: 52000 / Illumination mode: FLOOD BEAM / Imaging mode: BRIGHT FIELDBright-field microscopy / Nominal defocus max: 1.0 µm / Nominal defocus min: 1.0 µm / Nominal magnification: 46000
Sample stageSpecimen holder model: SIDE ENTRY, EUCENTRIC / Tilt angle max: 50
DateAug 15, 2015
Image recordingCategory: CCD / Film or detector model: TVIPS TEMCAM-F416 (4k x 4k) / Number real images: 119 / Average electron dose: 30.15 e/Å2
Tilt angle min0
Experimental equipment
Model: Tecnai Spirit / Image courtesy: FEI Company

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Image processing

Final two d classificationNumber classes: 104
Final reconstructionAlgorithm: OTHER / Resolution.type: BY AUTHOR / Resolution: 20.0 Å / Resolution method: OTHER / Software - Name: EMAN2, sparx / Number images used: 10706

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