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- EMDB-3003: Structure of adenovirus light particle Ad5/FC31 L2 -

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Basic information

Entry
Database: EMDB / ID: EMD-3003
TitleStructure of adenovirus light particle Ad5/FC31 L2
Map dataReconstruction of one type of light density particle (called L2) produced by adenovirus delayed packaging mutant Ad5/FC31
Sample
  • Sample: light density particle (called L2) produced by adenovirus delayed packaging mutant Ad5/FC31
  • Virus: Human adenovirus 5
Keywordsicosahedral virus assembly / adenovirus / virus maturation
Biological speciesHuman adenovirus 5
Methodsingle particle reconstruction / cryo EM / Resolution: 12.3 Å
AuthorsCondezo GN / Marabini R / Ayora S / Carazo JM / Chillon M / San Martin C
CitationJournal: J Virol / Year: 2015
Title: Structures of Adenovirus Incomplete Particles Clarify Capsid Architecture and Show Maturation Changes of Packaging Protein L1 52/55k.
Authors: Gabriela N Condezo / Roberto Marabini / Silvia Ayora / José M Carazo / Raúl Alba / Miguel Chillón / Carmen San Martín /
Abstract: Adenovirus is one of the most complex icosahedral, nonenveloped viruses. Even after its structure was solved at near-atomic resolution by both cryo-electron microscopy and X-ray crystallography, the ...Adenovirus is one of the most complex icosahedral, nonenveloped viruses. Even after its structure was solved at near-atomic resolution by both cryo-electron microscopy and X-ray crystallography, the location of minor coat proteins is still a subject of debate. The elaborated capsid architecture is the product of a correspondingly complex assembly process, about which many aspects remain unknown. Genome encapsidation involves the concerted action of five virus proteins, and proteolytic processing by the virus protease is needed to prime the virion for sequential uncoating. Protein L1 52/55k is required for packaging, and multiple cleavages by the maturation protease facilitate its release from the nascent virion. Light-density particles are routinely produced in adenovirus infections and are thought to represent assembly intermediates. Here, we present the molecular and structural characterization of two different types of human adenovirus light particles produced by a mutant with delayed packaging. We show that these particles lack core polypeptide V but do not lack the density corresponding to this protein in the X-ray structure, thereby adding support to the adenovirus cryo-electron microscopy model. The two types of light particles present different degrees of proteolytic processing. Their structures provide the first glimpse of the organization of L1 52/55k protein inside the capsid shell and of how this organization changes upon partial maturation. Immature, full-length L1 52/55k is poised beneath the vertices to engage the virus genome. Upon proteolytic processing, L1 52/55k disengages from the capsid shell, facilitating genome release during uncoating.
IMPORTANCE: Adenoviruses have been extensively characterized as experimental systems in molecular biology, as human pathogens, and as therapeutic vectors. However, a clear picture of many aspects of ...IMPORTANCE: Adenoviruses have been extensively characterized as experimental systems in molecular biology, as human pathogens, and as therapeutic vectors. However, a clear picture of many aspects of their basic biology is still lacking. Two of these aspects are the location of minor coat proteins in the capsid and the molecular details of capsid assembly. Here, we provide evidence supporting one of the two current models for capsid architecture. We also show for the first time the location of the packaging protein L1 52/55k in particles lacking the virus genome and how this location changes during maturation. Our results contribute to clarifying standing questions in adenovirus capsid architecture and provide new details on the role of L1 52/55k protein in assembly.
History
DepositionMay 8, 2015-
Header (metadata) releaseJun 17, 2015-
Map releaseJul 29, 2015-
UpdateSep 9, 2015-
Current statusSep 9, 2015Processing site: PDBe / Status: Released

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Structure visualization

Movie
  • Surface view with section colored by density value
  • Surface level: 1.4
  • Imaged by UCSF Chimera
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  • Surface view colored by radius
  • Surface level: 1.4
  • Imaged by UCSF Chimera
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Structure viewerEM map:
SurfViewMolmilJmol/JSmol
Supplemental images

Downloads & links

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Map

FileDownload / File: emd_3003.map.gz / Format: CCP4 / Size: 62.5 MB / Type: IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES)
AnnotationReconstruction of one type of light density particle (called L2) produced by adenovirus delayed packaging mutant Ad5/FC31
Voxel sizeX=Y=Z: 4.2 Å
Density
Contour LevelBy AUTHOR: 1.4 / Movie #1: 1.4
Minimum - Maximum-1.81644154 - 4.99485445
Average (Standard dev.)0.0 (±1.0)
SymmetrySpace group: 1
Details

EMDB XML:

Map geometry
Axis orderXYZ
Origin000
Dimensions256256256
Spacing256256256
CellA=B=C: 1075.2 Å
α=β=γ: 90.0 °

CCP4 map header:

modeImage stored as Reals
Å/pix. X/Y/Z4.24.24.2
M x/y/z256256256
origin x/y/z0.0000.0000.000
length x/y/z1075.2001075.2001075.200
α/β/γ90.00090.00090.000
start NX/NY/NZ-21-120
NX/NY/NZ432573
MAP C/R/S123
start NC/NR/NS000
NC/NR/NS256256256
D min/max/mean-1.8164.995-0.000

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Supplemental data

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Sample components

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Entire : light density particle (called L2) produced by adenovirus delayed...

EntireName: light density particle (called L2) produced by adenovirus delayed packaging mutant Ad5/FC31
Components
  • Sample: light density particle (called L2) produced by adenovirus delayed packaging mutant Ad5/FC31
  • Virus: Human adenovirus 5

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Supramolecule #1000: light density particle (called L2) produced by adenovirus delayed...

SupramoleculeName: light density particle (called L2) produced by adenovirus delayed packaging mutant Ad5/FC31
type: sample / ID: 1000 / Oligomeric state: icosahedral virus capsid / Number unique components: 1
Molecular weightTheoretical: 125 MDa

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Supramolecule #1: Human adenovirus 5

SupramoleculeName: Human adenovirus 5 / type: virus / ID: 1 / NCBI-ID: 28285 / Sci species name: Human adenovirus 5 / Virus type: VIRUS-LIKE PARTICLE / Virus isolate: SEROTYPE / Virus enveloped: No / Virus empty: Yes / Sci species serotype: 5
Host (natural)Organism: Homo sapiens (human) / synonym: VERTEBRATES
Molecular weightTheoretical: 150 MDa
Virus shellShell ID: 1 / Diameter: 950 Å / T number (triangulation number): 25

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Experimental details

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Structure determination

Methodcryo EM
Processingsingle particle reconstruction
Aggregation stateparticle

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Sample preparation

Concentration0.03 mg/mL
BufferpH: 7.8 / Details: 20 mm Hepes, 150 mm NaCl
GridDetails: Quantifoil R2/4 300 mesh Cu/Rh glow discharged grids
VitrificationCryogen name: ETHANE / Instrument: LEICA EM CPC

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Electron microscopy

MicroscopeFEI TECNAI F20
Electron beamAcceleration voltage: 200 kV / Electron source: FIELD EMISSION GUN
Electron opticsIllumination mode: FLOOD BEAM / Imaging mode: BRIGHT FIELDBright-field microscopy / Cs: 2.26 mm / Nominal defocus max: 4.0 µm / Nominal defocus min: 0.5 µm / Nominal magnification: 50000
Sample stageSpecimen holder model: GATAN LIQUID NITROGEN
DateNov 11, 2013
Image recordingCategory: CCD / Film or detector model: FEI EAGLE (4k x 4k) / Digitization - Sampling interval: 14 µm / Number real images: 889 / Average electron dose: 12 e/Å2
Experimental equipment
Model: Tecnai F20 / Image courtesy: FEI Company

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Image processing

CTF correctionDetails: micrograph
Final reconstructionApplied symmetry - Point group: I (icosahedral) / Algorithm: OTHER / Resolution.type: BY AUTHOR / Resolution: 12.3 Å / Resolution method: OTHER / Software - Name: Xmipp, Relion / Number images used: 6743
DetailsCTF detection, phase flip, particle selection, extraction and normalization with Xmipp. 2D and 3D classification, 3D refinement and postprocessing with Relion.

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Atomic model buiding 1

Initial modelPDB ID:

3iyn
PDB Unreleased entry

SoftwareName: Chimera
RefinementSpace: REAL / Protocol: RIGID BODY FIT / Target criteria: cross-correlation

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