histone catabolic process / CORVET complex / organelle fusion / HOPS complex / endosomal vesicle fusion / regulation of SNARE complex assembly / vesicle tethering / vacuole-mitochondrion membrane contact site / regulation of vacuole fusion, non-autophagic / vacuolar protein processing ...histone catabolic process / CORVET complex / organelle fusion / HOPS complex / endosomal vesicle fusion / regulation of SNARE complex assembly / vesicle tethering / vacuole-mitochondrion membrane contact site / regulation of vacuole fusion, non-autophagic / vacuolar protein processing / vacuole fusion, non-autophagic / Golgi to endosome transport / vesicle fusion with vacuole / Golgi to vacuole transport / cytoplasm to vacuole targeting by the Cvt pathway / vesicle docking / endosome organization / piecemeal microautophagy of the nucleus / protein targeting to vacuole / vacuole organization / fungal-type vacuole / vesicle docking involved in exocytosis / late endosome to vacuole transport / fungal-type vacuole membrane / vacuole / endosomal transport / vesicle-mediated transport / positive regulation of TORC1 signaling / cellular response to starvation / guanyl-nucleotide exchange factor activity / macroautophagy / intracellular protein transport / RING-type E3 ubiquitin transferase / small GTPase binding / autophagy / endocytosis / ubiquitin protein ligase activity / protein transport / protein-macromolecule adaptor activity / late endosome / actin binding / early endosome membrane / endosome / ATP binding / membrane / metal ion binding / cytosol / cytoplasm Similarity search - Function
Vacuolar protein sorting-associated protein 41 / Vacuolar protein sorting-associated protein 33 / Vacuolar protein sorting-associated protein Vps41/Vps8 / Pep3/Vps18/deep orange / Vacuolar protein sorting-associated protein 11 / Vacuolar sorting protein 39/Transforming growth factor beta receptor-associated domain 2 / Vacuolar protein sorting protein 11, C-terminal / Pep3/Vps18/deep orange beta-propeller domain / Vacuolar sorting protein 39 domain 2 / Vacuolar protein sorting protein 11 C terminal ...Vacuolar protein sorting-associated protein 41 / Vacuolar protein sorting-associated protein 33 / Vacuolar protein sorting-associated protein Vps41/Vps8 / Pep3/Vps18/deep orange / Vacuolar protein sorting-associated protein 11 / Vacuolar sorting protein 39/Transforming growth factor beta receptor-associated domain 2 / Vacuolar protein sorting protein 11, C-terminal / Pep3/Vps18/deep orange beta-propeller domain / Vacuolar sorting protein 39 domain 2 / Vacuolar protein sorting protein 11 C terminal / Vacuolar sorting protein 39/Transforming growth factor beta receptor-associated domain 1 / Vam6/VPS39/TRAP1 family / Vacuolar sorting protein 39 domain 1 / Vps16, C-terminal / Vps16, N-terminal / Vacuolar protein sorting-associated protein 16 / Vps16, C-terminal domain superfamily / Vps16, C-terminal region / Vps16, N-terminal region / Vacuolar protein sorting-associated protein 33, domain 3b / Sec1-like, domain 2 / Sec1-like, domain 3a / Sec1-like protein / Sec1-like superfamily / Sec1 family / Region in Clathrin and VPS / Clathrin heavy chain repeat homology / Clathrin, heavy chain/VPS, 7-fold repeat / Clathrin heavy-chain (CHCR) repeat profile. / Ring finger / Zinc finger, PHD-type / PHD zinc finger / Zinc finger RING-type profile. / Zinc finger, RING-type / Tetratricopeptide-like helical domain superfamily / Zinc finger, RING/FYVE/PHD-type / WD40 repeats / WD40 repeat / WD40-repeat-containing domain superfamily / WD40/YVTN repeat-like-containing domain superfamily Similarity search - Domain/homology
E3 ubiquitin-protein ligase PEP5 / Vacuolar protein sorting-associated protein 33 / Vacuolar membrane protein PEP3 / Vacuolar protein sorting-associated protein 41 / Vacuolar protein sorting-associated protein 16 / Vacuolar morphogenesis protein 6 Similarity search - Component
Biological species
Saccharomyces cerevisiae (brewer's yeast)
Method
single particle reconstruction / negative staining / Resolution: 30.4 Å
Journal: Proc Natl Acad Sci U S A / Year: 2012 Title: Molecular architecture of the multisubunit homotypic fusion and vacuole protein sorting (HOPS) tethering complex. Authors: Cornelia Bröcker / Anne Kuhlee / Christos Gatsogiannis / Henning J kleine Balderhaar / Carina Hönscher / Siegfried Engelbrecht-Vandré / Christian Ungermann / Stefan Raunser / Abstract: Membrane fusion within the eukaryotic endomembrane system depends on the initial recognition of Rab GTPase on transport vesicles by multisubunit tethering complexes and subsequent coupling to SNARE- ...Membrane fusion within the eukaryotic endomembrane system depends on the initial recognition of Rab GTPase on transport vesicles by multisubunit tethering complexes and subsequent coupling to SNARE-mediated fusion. The conserved vacuolar/lysosomal homotypic fusion and vacuole protein sorting (HOPS) tethering complex combines both activities. Here we present the overall structure of the fusion-active HOPS complex. Our data reveal a flexible ≈30-nm elongated seahorse-like structure, which can adopt contracted and elongated shapes. Surprisingly, both ends of the HOPS complex contain a Rab-binding subunit: Vps41 and Vps39. The large head contains in addition to Vps41 the SNARE-interacting Vps33, whereas Vps39 is found in the bulky tip of its tail. Vps11 and Vps18 connect head and tail. Our data suggest that HOPS bridges Ypt7-positive membranes and chaperones SNAREs at fusion sites.
History
Deposition
Jan 16, 2013
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Header (metadata) release
Jan 23, 2013
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Map release
Jan 23, 2013
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Update
Jan 23, 2013
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Current status
Jan 23, 2013
Processing site: PDBe / Status: Released
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Structure visualization
Movie
Surface view with section colored by density value
UniProtKB: Vacuolar morphogenesis protein 6 / GO: HOPS complex
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Macromolecule #3: vacuolar protein sorting-associated protein 33
Macromolecule
Name: vacuolar protein sorting-associated protein 33 / type: protein_or_peptide / ID: 3 Name.synonym: Protein SLP1, Vacuolar morphogenesis protein 5, vps33 Number of copies: 1 / Oligomeric state: Monomer / Recombinant expression: No
Category: FILM / Film or detector model: KODAK SO-163 FILM / Digitization - Scanner: OTHER / Digitization - Sampling interval: 10.5 µm / Number real images: 920 / Average electron dose: 4.5 e/Å2 / Bits/pixel: 16
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Image processing
Final reconstruction
Applied symmetry - Point group: C1 (asymmetric) / Algorithm: OTHER / Resolution.type: BY AUTHOR / Resolution: 30.4 Å / Resolution method: FSC 0.5 CUT-OFF / Software - Name: SPARX / Number images used: 2979
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