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Yorodumi- PDB-1qgh: THE X-RAY STRUCTURE OF THE UNUSUAL DODECAMERIC FERRITIN FROM LIST... -
+Open data
-Basic information
Entry | Database: PDB / ID: 1qgh | ||||||
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Title | THE X-RAY STRUCTURE OF THE UNUSUAL DODECAMERIC FERRITIN FROM LISTERIA INNOCUA, REVEALS A NOVEL INTERSUBUNIT IRON BINDING SITE. | ||||||
Components | NON-HEME IRON-CONTAINING FERRITIN | ||||||
Keywords | METAL TRANSPORT / FERRITIN | ||||||
Function / homology | Function and homology information Oxidoreductases; Oxidizing metal ions / oxidoreductase activity, acting on metal ions / ferric iron binding / intracellular iron ion homeostasis / cytoplasm Similarity search - Function | ||||||
Biological species | Listeria innocua (bacteria) | ||||||
Method | X-RAY DIFFRACTION / SYNCHROTRON / MOLECULAR REPLACEMENT / Resolution: 2.35 Å | ||||||
Authors | Ilari, A. / Stefanini, S. / Chiancone, E. / Tsernoglou, D. | ||||||
Citation | Journal: Nat.Struct.Biol. / Year: 2000 Title: The dodecameric ferritin from Listeria innocua contains a novel intersubunit iron-binding site. Authors: Ilari, A. / Stefanini, S. / Chiancone, E. / Tsernoglou, D. | ||||||
History |
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-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
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-Downloads & links
-Download
PDBx/mmCIF format | 1qgh.cif.gz | 371.8 KB | Display | PDBx/mmCIF format |
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PDB format | pdb1qgh.ent.gz | 302.7 KB | Display | PDB format |
PDBx/mmJSON format | 1qgh.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/qg/1qgh ftp://data.pdbj.org/pub/pdb/validation_reports/qg/1qgh | HTTPS FTP |
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-Related structure data
Related structure data | 1dpsS S: Starting model for refinement |
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Similar structure data |
-Links
-Assembly
Deposited unit |
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Unit cell |
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Noncrystallographic symmetry (NCS) | NCS oper:
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-Components
#1: Protein | Mass: 18070.553 Da / Num. of mol.: 12 / Source method: isolated from a natural source / Source: (natural) Listeria innocua (bacteria) / References: UniProt: P80725 #2: Chemical | ChemComp-FE / #3: Water | ChemComp-HOH / | |
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-Experimental details
-Experiment
Experiment | Method: X-RAY DIFFRACTION / Number of used crystals: 1 |
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-Sample preparation
Crystal | Density Matthews: 2.41 Å3/Da / Density % sol: 48.86 % | ||||||||||||||||||||
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Crystal grow | pH: 5.8 Details: PEG 1000 AT 19-25 %, MES 0.1 M AT PH 5.0-6.2, pH 5.8 | ||||||||||||||||||||
Crystal grow | *PLUS Temperature: 293 K / Method: vapor diffusion, sitting drop | ||||||||||||||||||||
Components of the solutions | *PLUS
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-Data collection
Diffraction | Mean temperature: 100 K |
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Diffraction source | Source: SYNCHROTRON / Site: EMBL/DESY, HAMBURG / Beamline: BW7B / Wavelength: 0.8373 |
Detector | Date: Dec 1, 1997 |
Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 0.8373 Å / Relative weight: 1 |
Reflection | Resolution: 2.35→30 Å / Num. obs: 80150 / % possible obs: 91.3 % / Redundancy: 3.8 % / Rsym value: 0.077 |
Reflection | *PLUS Rmerge(I) obs: 0.077 |
-Processing
Software |
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Refinement | Method to determine structure: MOLECULAR REPLACEMENT Starting model: 1DPS Resolution: 2.35→10 Å / σ(F): 0
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Refinement step | Cycle: LAST / Resolution: 2.35→10 Å
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Refine LS restraints |
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Software | *PLUS Name: TNT / Version: 5E / Classification: refinement | ||||||||||||||||||||||||||||||
Refine LS restraints | *PLUS
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