+データを開く
-基本情報
登録情報 | データベース: SASBDB / ID: SASDEW6 |
---|---|
試料 | KRAB-associated protein 1, (KAP1); TRIM28; 23-418 RBCC domain
|
機能・相同性 | 機能・相同性情報 convergent extension involved in axis elongation / : / Krueppel-associated box domain binding / embryonic placenta morphogenesis / positive regulation of DNA methylation-dependent heterochromatin formation / suppression of viral release by host / epigenetic programming of gene expression / negative regulation of single stranded viral RNA replication via double stranded DNA intermediate / ゲノム刷り込み / chromo shadow domain binding ...convergent extension involved in axis elongation / : / Krueppel-associated box domain binding / embryonic placenta morphogenesis / positive regulation of DNA methylation-dependent heterochromatin formation / suppression of viral release by host / epigenetic programming of gene expression / negative regulation of single stranded viral RNA replication via double stranded DNA intermediate / ゲノム刷り込み / chromo shadow domain binding / Generic Transcription Pathway / SUMO transferase activity / protein sumoylation / 上皮間葉転換 / ヘテロクロマチン / 着床 / positive regulation of DNA repair / SUMOylation of transcription cofactors / promoter-specific chromatin binding / ユークロマチン / RING-type E3 ubiquitin transferase / HCMV Early Events / positive regulation of protein import into nucleus / RNA polymerase II transcription regulator complex / ubiquitin-protein transferase activity / transcription corepressor activity / ubiquitin protein ligase activity / chromatin organization / transcription coactivator activity / protein kinase activity / DNA修復 / 自然免疫系 / negative regulation of DNA-templated transcription / ubiquitin protein ligase binding / chromatin binding / positive regulation of DNA-templated transcription / negative regulation of transcription by RNA polymerase II / protein-containing complex / DNA binding / RNA binding / zinc ion binding / 核質 / 細胞核 類似検索 - 分子機能 |
生物種 | Homo sapiens (ヒト) |
引用 | ジャーナル: Life Sci Alliance / 年: 2019 タイトル: KAP1 is an antiparallel dimer with a functional asymmetry. 著者: Giulia Fonti / Maria J Marcaida / Louise C Bryan / Sylvain Träger / Alexandra S Kalantzi / Pierre-Yves Jl Helleboid / Davide Demurtas / Mark D Tully / Sergei Grudinin / Didier Trono / Beat ...著者: Giulia Fonti / Maria J Marcaida / Louise C Bryan / Sylvain Träger / Alexandra S Kalantzi / Pierre-Yves Jl Helleboid / Davide Demurtas / Mark D Tully / Sergei Grudinin / Didier Trono / Beat Fierz / Matteo Dal Peraro / 要旨: KAP1 (KRAB domain-associated protein 1) plays a fundamental role in regulating gene expression in mammalian cells by recruiting different transcription factors and altering the chromatin state. In ...KAP1 (KRAB domain-associated protein 1) plays a fundamental role in regulating gene expression in mammalian cells by recruiting different transcription factors and altering the chromatin state. In doing so, KAP1 acts both as a platform for macromolecular interactions and as an E3 small ubiquitin modifier ligase. This work sheds light on the overall organization of the full-length protein combining solution scattering data, integrative modeling, and single-molecule experiments. We show that KAP1 is an elongated antiparallel dimer with an asymmetry at the C-terminal domains. This conformation is consistent with the finding that the Really Interesting New Gene (RING) domain contributes to KAP1 auto-SUMOylation. Importantly, this intrinsic asymmetry has key functional implications for the KAP1 network of interactions, as the heterochromatin protein 1 (HP1) occupies only one of the two putative HP1 binding sites on the KAP1 dimer, resulting in an unexpected stoichiometry, even in the context of chromatin fibers. |
登録者 |
|
-構造の表示
構造ビューア | 分子: MolmilJmol/JSmol |
---|
-ダウンロードとリンク
-モデル
モデル #2559 | タイプ: dummy / ダミー原子の半径: 1.90 A / 対称性: p1 / カイ2乗値: 0.98 / P-value: 0.000034 Omokage検索でこの集合体の類似形状データを探す (詳細) |
---|---|
モデル #2560 | タイプ: atomic / 対称性: p1 / P-value: 0.017702 Omokage検索でこの集合体の類似形状データを探す (詳細) |
-試料
試料 | 名称: KRAB-associated protein 1, (KAP1); TRIM28; 23-418 RBCC domain 試料濃度: 12 mg/ml |
---|---|
バッファ | 名称: 20 mM HEPES, 500 mM NaCl, 10 % Glycerol, 2 mM TCEP / pH: 7.5 |
要素 #1352 | 名称: RBCC DOMAIN KAP1 / タイプ: protein 記述: Transcription intermediary factor 1-beta, TIF1b, KAP1, TRIM28, Fragment 23-418, RBCC domain 分子量: 46.135 / 分子数: 2 / 由来: Homo sapiens / 参照: UniProt: Q13263 配列: MGSSHHHHHH SQDPNSSSEN LYFQGEGSAG GEKRSTAPSA AASASASAAA SSPAGGGAEA LELLEHCGVC RERLRPEREP RLLPCLHSAC SACLGPAAPA AANSSGDGGA AGDGTVVDCP VCKQQCFSKD IVENYFMRDS GSKAATDAQD ANQCCTSCED NAPATSYCVE ...配列: MGSSHHHHHH SQDPNSSSEN LYFQGEGSAG GEKRSTAPSA AASASASAAA SSPAGGGAEA LELLEHCGVC RERLRPEREP RLLPCLHSAC SACLGPAAPA AANSSGDGGA AGDGTVVDCP VCKQQCFSKD IVENYFMRDS GSKAATDAQD ANQCCTSCED NAPATSYCVE CSEPLCETCV EAHQRVKYTK DHTVRSTGPA KSRDGERTVY CNVHKHEPLV LFCESCDTLT CRDCQLNAHK DHQYQFLEDA VRNQRKLLAS LVKRLGDKHA TLQKSTKEVR SSIRQVSDVQ KRVQVDVKMA ILQIMKELNK RGRVLVNDAQ KVTEGQQERL ERQHWTMTKI QKHQEHILRF ASWALESDNN TALLLSKKLI YFQLHRALKM IVDPVEPHGE MKFQWDLNAW TKSAEAFGKI VAERPGTNS |
-実験情報
ビーム | 設備名称: ESRF BM29 / 地域: Grenoble / 国: France / 線源: X-ray synchrotronシンクロトロン / 波長: 0.099 Å / スペクトロメータ・検出器間距離: 2.867 mm | ||||||||||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
検出器 | 名称: Pilatus 1M / タイプ: Dectris / Pixsize x: 172 mm | ||||||||||||||||||||||||||||||
スキャン | タイトル: KRAB-associated protein 1, (KAP1); TRIM28; 23-418 RBCC domain 測定日: 2017年7月6日 / 保管温度: 20 °C / セル温度: 20 °C / 照射時間: 1 sec. / 単位: 1/nm /
| ||||||||||||||||||||||||||||||
距離分布関数 P(R) | ソフトウェア P(R): GNOM 5.0 / ポイント数: 598 /
| ||||||||||||||||||||||||||||||
結果 | カーブのタイプ: sec /
|